Abstract
Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for ∼14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.
| Original language | English |
|---|---|
| Pages (from-to) | 974-983 |
| Number of pages | 10 |
| Journal | Journal of Genetics and Genomics |
| Volume | 34 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2007 |
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All Science Journal Classification (ASJC) codes
- Genetics
- Molecular Biology
Cite this
Effect of Menopause on Gene Expression Profiles of Circulating Monocytes : A Pilot in vivo Microarray Study. / Dvornyk, Volodymyr; Liu, Yaozhong; Lu, Yan; Shen, Hui; Lappe, Joan M.; Recker, Robert R.; Deng, Hongwen; Lei, Shufeng.
In: Journal of Genetics and Genomics, Vol. 34, No. 11, 11.2007, p. 974-983.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Menopause on Gene Expression Profiles of Circulating Monocytes
T2 - A Pilot in vivo Microarray Study
AU - Dvornyk, Volodymyr
AU - Liu, Yaozhong
AU - Lu, Yan
AU - Shen, Hui
AU - Lappe, Joan M.
AU - Recker, Robert R.
AU - Deng, Hongwen
AU - Lei, Shufeng
PY - 2007/11
Y1 - 2007/11
N2 - Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for ∼14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.
AB - Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for ∼14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.
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U2 - 10.1016/S1673-8527(07)60110-6
DO - 10.1016/S1673-8527(07)60110-6
M3 - Article
VL - 34
SP - 974
EP - 983
JO - Journal of Genetics and Genomics
JF - Journal of Genetics and Genomics
SN - 1673-8527
IS - 11
ER -