Abstract
In a previous study, we showed that hydrogen peroxide (H2O2) enhanced adrenergic neurosecretion in irides from several mammalian species presumably via a prejunctional action (Ohia et al., Exp. Eye Res. 59:Abs. No. 173, 1994). Purpose: To examine the effect of stress caused by various oxidants on norepinephrine (NE) release and membrane lipid peroxidation in the bovine isolated iris. Methods: Hemi-rides were isolated from bovine eyes and prepared for [3H]NE release assays using the superfusion method. For biochemical assays, lipid peroxidation was measured in iris membranes by determining their content of thiobarbituric acid reactive substances in the absence and presence of oxidants. Results: H2O2, cumene hydroperoxide (cuOOH) and t-butyl hydroperoxide (buOOH) caused a concentration-related enhancement of field stimulation-induced [3H]NE overflow with the following rank of potency: cuOOH>H2O2>buOOH. Potentiation of evoked [3H]NE release produced by submaximal concentrations of H2O2 (0.3 mM) and cuOOH (0.03 mM) was abolished by the antioxidant, melatonin (2 mM). Both H2O2 and cuOOH caused a concentration-dependent increase in lipid peroxidation over basal levels in isolated iris membranes. For instance, cuOOH (0.3 mM) increased lipid peroxidation (nmoles MDA/mg protein) from 2.99 ± 0.11 (n=10) to 8.16 ± 0.37 (n=7). Conclusion: We conclude that oxidants produce a specific enhancement of evoked NE release from sympathetic nerves in the bovine iris probably via an effect on membrane lipid peroxidation.
| Original language | English |
|---|---|
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 3 |
| State | Published - Feb 15 1996 |
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All Science Journal Classification (ASJC) codes
- Ophthalmology
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Effect of oxidative stress on sympathetic neurotransmission in the bovine iris. / Opere, Catherine A.; Imler, M.; Kim, J.; Ohia, S. E.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of oxidative stress on sympathetic neurotransmission in the bovine iris
AU - Opere, Catherine A.
AU - Imler, M.
AU - Kim, J.
AU - Ohia, S. E.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - In a previous study, we showed that hydrogen peroxide (H2O2) enhanced adrenergic neurosecretion in irides from several mammalian species presumably via a prejunctional action (Ohia et al., Exp. Eye Res. 59:Abs. No. 173, 1994). Purpose: To examine the effect of stress caused by various oxidants on norepinephrine (NE) release and membrane lipid peroxidation in the bovine isolated iris. Methods: Hemi-rides were isolated from bovine eyes and prepared for [3H]NE release assays using the superfusion method. For biochemical assays, lipid peroxidation was measured in iris membranes by determining their content of thiobarbituric acid reactive substances in the absence and presence of oxidants. Results: H2O2, cumene hydroperoxide (cuOOH) and t-butyl hydroperoxide (buOOH) caused a concentration-related enhancement of field stimulation-induced [3H]NE overflow with the following rank of potency: cuOOH>H2O2>buOOH. Potentiation of evoked [3H]NE release produced by submaximal concentrations of H2O2 (0.3 mM) and cuOOH (0.03 mM) was abolished by the antioxidant, melatonin (2 mM). Both H2O2 and cuOOH caused a concentration-dependent increase in lipid peroxidation over basal levels in isolated iris membranes. For instance, cuOOH (0.3 mM) increased lipid peroxidation (nmoles MDA/mg protein) from 2.99 ± 0.11 (n=10) to 8.16 ± 0.37 (n=7). Conclusion: We conclude that oxidants produce a specific enhancement of evoked NE release from sympathetic nerves in the bovine iris probably via an effect on membrane lipid peroxidation.
AB - In a previous study, we showed that hydrogen peroxide (H2O2) enhanced adrenergic neurosecretion in irides from several mammalian species presumably via a prejunctional action (Ohia et al., Exp. Eye Res. 59:Abs. No. 173, 1994). Purpose: To examine the effect of stress caused by various oxidants on norepinephrine (NE) release and membrane lipid peroxidation in the bovine isolated iris. Methods: Hemi-rides were isolated from bovine eyes and prepared for [3H]NE release assays using the superfusion method. For biochemical assays, lipid peroxidation was measured in iris membranes by determining their content of thiobarbituric acid reactive substances in the absence and presence of oxidants. Results: H2O2, cumene hydroperoxide (cuOOH) and t-butyl hydroperoxide (buOOH) caused a concentration-related enhancement of field stimulation-induced [3H]NE overflow with the following rank of potency: cuOOH>H2O2>buOOH. Potentiation of evoked [3H]NE release produced by submaximal concentrations of H2O2 (0.3 mM) and cuOOH (0.03 mM) was abolished by the antioxidant, melatonin (2 mM). Both H2O2 and cuOOH caused a concentration-dependent increase in lipid peroxidation over basal levels in isolated iris membranes. For instance, cuOOH (0.3 mM) increased lipid peroxidation (nmoles MDA/mg protein) from 2.99 ± 0.11 (n=10) to 8.16 ± 0.37 (n=7). Conclusion: We conclude that oxidants produce a specific enhancement of evoked NE release from sympathetic nerves in the bovine iris probably via an effect on membrane lipid peroxidation.
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UR - http://www.scopus.com/inward/citedby.url?scp=1842292002&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:1842292002
VL - 37
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 3
ER -