Abstract
In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.
| Original language | English |
|---|---|
| Pages (from-to) | 811-818 |
| Number of pages | 8 |
| Journal | Neurochemical Research |
| Volume | 29 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2004 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Biochemistry
Cite this
Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae. / LeDay, Angela M.; Awe, Sunday O.; Kulkarni, Kaustubh; Harris, Lydia C.; Opere, Catherine A.; Dash, Alekha K.; Ohia, Sunny E.
In: Neurochemical Research, Vol. 29, No. 4, 04.2004, p. 811-818.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae
AU - LeDay, Angela M.
AU - Awe, Sunday O.
AU - Kulkarni, Kaustubh
AU - Harris, Lydia C.
AU - Opere, Catherine A.
AU - Dash, Alekha K.
AU - Ohia, Sunny E.
PY - 2004/4
Y1 - 2004/4
N2 - In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.
AB - In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.
UR - http://www.scopus.com/inward/record.url?scp=1842455060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842455060&partnerID=8YFLogxK
U2 - 10.1023/B:NERE.0000018855.77589.5d
DO - 10.1023/B:NERE.0000018855.77589.5d
M3 - Article
C2 - 15098946
AN - SCOPUS:1842455060
VL - 29
SP - 811
EP - 818
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 4
ER -