Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae

Angela M. LeDay; Sunday O. Awe; Kaustubh Kulkarni; Lydia C. Harris; Catherine Opere; Alekha Dash; Sunny E. Ohia

doi:10.1023/B:NERE.0000018855.77589.5d

Effect of Peroxides on [³H]D-Aspartate Release from Bovine Isolated Retinae

Angela M. LeDay, Sunday O. Awe, Kaustubh Kulkarni, Lydia C. Harris, Catherine Opere, Alekha Dash, Sunny E. Ohia

Department of Pharmacy Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K⁺-depolarization-evoked release of [ ³H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H₂O₂) ex vivo. Both naturally occurring H ₂O₂ (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K⁺-evoked [³H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [³H]D-aspartate overflow elicited by both H₂O ₂ (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H₂O₂ (1 μM) but antagonized the effect of H₂O₂ (30 μM) on K⁺-induced [ ³H]D-aspartate release. In ex vivo experiments, exogenously applied H₂O₂ (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K⁺-evoked release of [³H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.

Original language	English (US)
Pages (from-to)	811-818
Number of pages	8
Journal	Neurochemical Research
Volume	29
Issue number	4
DOIs	https://doi.org/10.1023/B:NERE.0000018855.77589.5d
State	Published - Apr 2004

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience

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@article{6e755db15e5043e3a08725173ee6f45a,

title = "Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae",

abstract = "In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.",

author = "LeDay, {Angela M.} and Awe, {Sunday O.} and Kaustubh Kulkarni and Harris, {Lydia C.} and Catherine Opere and Alekha Dash and Ohia, {Sunny E.}",

note = "Funding Information: The authors thank Greater Omaha Packing Company and Nebraska Beef Company for their generous donation of cow eyeballs. The authors also thank Ms. Kathy Widman (Creighton University) and Ms. Angela Clifton (University of Houston) for their excellent secretarial assistance. This project was supported by Grant Number EY13266 from the National Institutes of Health to Dr. Sunny E. Ohia.",

year = "2004",

month = apr,

doi = "10.1023/B:NERE.0000018855.77589.5d",

language = "English (US)",

volume = "29",

pages = "811--818",

journal = "Neurochemical Research",

issn = "0364-3190",

publisher = "Springer New York",

number = "4",

}

TY - JOUR

T1 - Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae

AU - LeDay, Angela M.

AU - Awe, Sunday O.

AU - Kulkarni, Kaustubh

AU - Harris, Lydia C.

AU - Opere, Catherine

AU - Dash, Alekha

AU - Ohia, Sunny E.

N1 - Funding Information: The authors thank Greater Omaha Packing Company and Nebraska Beef Company for their generous donation of cow eyeballs. The authors also thank Ms. Kathy Widman (Creighton University) and Ms. Angela Clifton (University of Houston) for their excellent secretarial assistance. This project was supported by Grant Number EY13266 from the National Institutes of Health to Dr. Sunny E. Ohia.

PY - 2004/4

Y1 - 2004/4

N2 - In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.

AB - In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.

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U2 - 10.1023/B:NERE.0000018855.77589.5d

DO - 10.1023/B:NERE.0000018855.77589.5d

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AN - SCOPUS:1842455060

VL - 29

SP - 811

EP - 818

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 4