Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae

Angela M. LeDay, Sunday O. Awe, Kaustubh Kulkarni, Lydia C. Harris, Catherine A. Opere, Alekha K. Dash, Sunny E. Ohia

Research output: Contribution to journalArticle

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Abstract

In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [ 3H]D-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2) ex vivo. Both naturally occurring H 2O2 (1-100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1-100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]D-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]D-aspartate overflow elicited by both H2O 2 (30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2 (1 μM) but antagonized the effect of H2O2 (30 μM) on K+-induced [ 3H]D-aspartate release. In ex vivo experiments, exogenously applied H2O2 (1-100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]D-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.

Original languageEnglish
Pages (from-to)811-818
Number of pages8
JournalNeurochemical Research
Volume29
Issue number4
DOIs
StatePublished - Apr 2004

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D-Aspartic Acid
Peroxides
Retina
Glutamic Acid
Vitreous Body
Triazoles
Tritium
Depolarization
Catalase
Hydrogen Peroxide
Antioxidants
High Pressure Liquid Chromatography
Experiments

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

Cite this

Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae. / LeDay, Angela M.; Awe, Sunday O.; Kulkarni, Kaustubh; Harris, Lydia C.; Opere, Catherine A.; Dash, Alekha K.; Ohia, Sunny E.

In: Neurochemical Research, Vol. 29, No. 4, 04.2004, p. 811-818.

Research output: Contribution to journalArticle

LeDay, Angela M. ; Awe, Sunday O. ; Kulkarni, Kaustubh ; Harris, Lydia C. ; Opere, Catherine A. ; Dash, Alekha K. ; Ohia, Sunny E. / Effect of Peroxides on [3H]D-Aspartate Release from Bovine Isolated Retinae. In: Neurochemical Research. 2004 ; Vol. 29, No. 4. pp. 811-818.
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