Effect of platelet-activating factor on beta-adrenoceptors in human lung

Devendra K. Agrawal, Robert G. Townley

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We examined the effect of platelet-activating factor (PAF) on beta-adrenoceptors in the membranes of human lung and the functional responses to methacholine, histamine and isoproterenol in human lung parenchyma and tracheal strips. Preincubation of the human lung slices with 0.1 uM PAF decreased the density of beta-adrenoceptors without a change in the affinity. In the functional studies, a subthreshold dose of PAF (0.1 uM) significantly reduced the potency of isoproterenol to reverse methacholine or histamine-induced contraction. BN 52021, a PAF antagonist, protected against these PAF-induced changes. These findings suggest that PAF-induced down-regulation of beta-adrenoceptors could be a mechanism of the non-specific airway hyperreactivity in asthma.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume143
Issue number1
DOIs
StatePublished - Feb 27 1987

Fingerprint

Platelet Activating Factor
Adrenergic Receptors
Lung
ginkgolide B
Methacholine Chloride
Isoproterenol
Histamine
Down-Regulation
Asthma
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of platelet-activating factor on beta-adrenoceptors in human lung. / Agrawal, Devendra K.; Townley, Robert G.

In: Biochemical and Biophysical Research Communications, Vol. 143, No. 1, 27.02.1987, p. 1-6.

Research output: Contribution to journalArticle

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