Effect of platelet-activating factor on beta-adrenoceptors in human lung

Devendra K. Agrawal; Robert G. Townley

doi:10.1016/0006-291X(87)90620-6

Effect of platelet-activating factor on beta-adrenoceptors in human lung

Devendra K. Agrawal, Robert G. Townley

Department of Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We examined the effect of platelet-activating factor (PAF) on beta-adrenoceptors in the membranes of human lung and the functional responses to methacholine, histamine and isoproterenol in human lung parenchyma and tracheal strips. Preincubation of the human lung slices with 0.1 uM PAF decreased the density of beta-adrenoceptors without a change in the affinity. In the functional studies, a subthreshold dose of PAF (0.1 uM) significantly reduced the potency of isoproterenol to reverse methacholine or histamine-induced contraction. BN 52021, a PAF antagonist, protected against these PAF-induced changes. These findings suggest that PAF-induced down-regulation of beta-adrenoceptors could be a mechanism of the non-specific airway hyperreactivity in asthma.

Original language	English (US)
Pages (from-to)	1-6
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	143
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(87)90620-6
State	Published - Feb 27 1987

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "We examined the effect of platelet-activating factor (PAF) on beta-adrenoceptors in the membranes of human lung and the functional responses to methacholine, histamine and isoproterenol in human lung parenchyma and tracheal strips. Preincubation of the human lung slices with 0.1 uM PAF decreased the density of beta-adrenoceptors without a change in the affinity. In the functional studies, a subthreshold dose of PAF (0.1 uM) significantly reduced the potency of isoproterenol to reverse methacholine or histamine-induced contraction. BN 52021, a PAF antagonist, protected against these PAF-induced changes. These findings suggest that PAF-induced down-regulation of beta-adrenoceptors could be a mechanism of the non-specific airway hyperreactivity in asthma.",

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Effect of platelet-activating factor on beta-adrenoceptors in human lung

Abstract

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