Effect of prostaglandins on cyclic AMP production in cultured human ciliary muscle cells

G. L. Zhan, C. B. Camras, Catherine A. Opere, L. Tang, S. E. Ohia

Research output: Contribution to journalArticle

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Abstract

Prostaglandins (PGs) lower intraocular pressure by increasing uveoscleral outflow, presumably via a receptor-mediated mechanism coupled to a second messenger pathway in the ciliary muscle. In the present study, we examined the effect of prostanoids on cyclic AMP production in cultured human ciliary muscle cells. Cells were identified based on their expression of smooth muscle specific α-actin and monoclonal antibody against desmin. Cyclic AMP production in confluent cells incubated with buffer solution containing various concentrations of prostanoids was analyzed by radioimmunoassay. PGE 2 caused a time-dependent increase in cyclic AMP concentrations which reached a maximum after 10 mins. With the exception of PGD 2, all prostanoids produced a concentration-dependent increase in cyclic AMP levels with the following rank order of activity: PGE 2 > 11-deoxy-PGE 1 > 16,16-dimethyl PGE 2 > sulprostone > PGF(2α). PGE 2-induced increase on cyclic AMP levels was unaffected by AH6809, an antagonist at both PGD 2 (DP) and E 2 (EP 1) receptors. Flurbiprofen decreased basal cyclic AMP concentrations suggesting that intramurally-generated PGs stimulate the formation of the nucleotide in ciliary smooth muscle cells. PGE 2-induced increases in cyclic AMP production was synergistic with those induced by the diterpene activator of adenylyl cyclase, forskolin. We conclude that prostanoids active at EP 2-receptors can stimulate cyclic AMP production in cultured human ciliary muscle cells.

Original languageEnglish
Pages (from-to)45-55
Number of pages11
JournalJournal of Ocular Pharmacology and Therapeutics
Volume14
Issue number1
StatePublished - Feb 1998

Fingerprint

Cyclic AMP
Muscle Cells
Prostaglandins
Prostaglandins E
Prostaglandins D
Flurbiprofen
Desmin
Diterpenes
Prostaglandins F
Second Messenger Systems
Colforsin
Intraocular Pressure
Adenylyl Cyclases
Smooth Muscle Myocytes
Radioimmunoassay
Smooth Muscle
Actins
Buffers
Nucleotides
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effect of prostaglandins on cyclic AMP production in cultured human ciliary muscle cells. / Zhan, G. L.; Camras, C. B.; Opere, Catherine A.; Tang, L.; Ohia, S. E.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 14, No. 1, 02.1998, p. 45-55.

Research output: Contribution to journalArticle

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