TY - JOUR
T1 - Effects of hypergravity on the prenatal development of peripheral vestibulocerebellar afferent fibers
AU - Bruce, L. L.
AU - Burke, J. M.
AU - Dobrowolska, J. A.
N1 - Funding Information:
We thank April Ronca for inviting us to participate in the hypergravity study at Ames Research Center. We also thank Bernd Fritzsch for reading the manuscript, Michael Kling for his technical help in this study, and the Nebraska Center for Cell Biology for support of the confocal microscope. This project was supported by NASA Grant NAG2-1595, and by NIH Grant 5P20 RR-16469 from the BRIN Program of the National Center for Research Resources.
PY - 2006
Y1 - 2006
N2 - The relationship between vestibular stimulation and the distribution of peripheral vestibulocerebellar sensory fibers was studied in embryonic rats that developed in normal gravity (1G), 1.75G, 2.0G, or rotational environments from 10 to 20 days of gestation. Subsequently a fluorescent neuronal tracer was applied to the cerebellum, and allowed to diffuse retrogradely to the vestibular periphery. The distribution of labeled fibers and terminals in the posterior vertical canal and the utricle was analyzed. Sensory fibers in the rotation- and hypergravity-exposed embryos of the posterior semicircular canal and utricle displayed fewer long extending fibers and more terminal fields, suggesting faster rates of maturation as compared to the synchronous controls. Hypergravity exposures in the posterior canal caused increased terminal formation in the central zone of the cristae, and in the utricle caused increased terminal formation, including calyces, in the medial extrastriolar zone. These results show the importance of the vestibular environment in the development of peripheral vestibular innervation.
AB - The relationship between vestibular stimulation and the distribution of peripheral vestibulocerebellar sensory fibers was studied in embryonic rats that developed in normal gravity (1G), 1.75G, 2.0G, or rotational environments from 10 to 20 days of gestation. Subsequently a fluorescent neuronal tracer was applied to the cerebellum, and allowed to diffuse retrogradely to the vestibular periphery. The distribution of labeled fibers and terminals in the posterior vertical canal and the utricle was analyzed. Sensory fibers in the rotation- and hypergravity-exposed embryos of the posterior semicircular canal and utricle displayed fewer long extending fibers and more terminal fields, suggesting faster rates of maturation as compared to the synchronous controls. Hypergravity exposures in the posterior canal caused increased terminal formation in the central zone of the cristae, and in the utricle caused increased terminal formation, including calyces, in the medial extrastriolar zone. These results show the importance of the vestibular environment in the development of peripheral vestibular innervation.
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U2 - 10.1016/j.asr.2006.03.002
DO - 10.1016/j.asr.2006.03.002
M3 - Article
AN - SCOPUS:33750505679
VL - 38
SP - 1041
EP - 1051
JO - Advances in Space Research
JF - Advances in Space Research
SN - 0273-1177
IS - 6
ER -