Effects of nicotine on bone and calciotropic hormones in growing female rats

U. T. Iwaniec, Y. K. Fung, D. M. Cullen, Mohammed P. Akhter, M. C. Haven, M. Schmid

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Limited research in young adults and immature animals suggests a detrimental effect of tobacco on bone during growth. This study investigated the effects of nicotine, the major alkaloid component of tobacco, on calciotropic hormone concentrations and bone status in growing female rats. One-month-old animals received either saline (n = 10), nicotine at 3.0 mg/kg/day (n = 10), or nicotine at 4.5 mg/kg/day (n = 10) administered subcutaneously via osmotic minipumps for either 2 or 3 months. Sera, femora, tibiae, and lumbar vertebrae (3-5) were collected at necropsy. The concentrations of serum calcium, phosphorus, 25-hydroxyvitamin D, 1,25- dihydroxyvitamin D, parathyroid hormone, calcitonin, and insulin-like growth factor-I were determined. Bone variables evaluated included mineral content and density (vertebrae and femora), cancellous and cortical histomorphometry (tibiae), and bone strength (vertebrae and femora). Statistically significant differences in serum mineral and hormone concentrations were not associated with nicotine dose or exposure time. No significant nicotine treatment effects were detected for bone mineral content and density, bone histomorphometry, or bone strength. We conclude that nicotine treatment for 2 or 3 months at serum concentrations in the upper range of those found in smokers has no detrimental effect on bone mass, volume, or strength in the growing rat.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalCalcified Tissue International
Volume67
Issue number1
StatePublished - 2000

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Nicotine
Hormones
Bone and Bones
Femur
Serum
Tibia
Bone Density
Tobacco
Minerals
Spine
Lumbar Vertebrae
Bone Development
Calcitonin
Parathyroid Hormone
Insulin-Like Growth Factor I
Alkaloids
Phosphorus
Young Adult
Calcium
Therapeutics

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Iwaniec, U. T., Fung, Y. K., Cullen, D. M., Akhter, M. P., Haven, M. C., & Schmid, M. (2000). Effects of nicotine on bone and calciotropic hormones in growing female rats. Calcified Tissue International, 67(1), 68-74.

Effects of nicotine on bone and calciotropic hormones in growing female rats. / Iwaniec, U. T.; Fung, Y. K.; Cullen, D. M.; Akhter, Mohammed P.; Haven, M. C.; Schmid, M.

In: Calcified Tissue International, Vol. 67, No. 1, 2000, p. 68-74.

Research output: Contribution to journalArticle

Iwaniec, UT, Fung, YK, Cullen, DM, Akhter, MP, Haven, MC & Schmid, M 2000, 'Effects of nicotine on bone and calciotropic hormones in growing female rats', Calcified Tissue International, vol. 67, no. 1, pp. 68-74.
Iwaniec, U. T. ; Fung, Y. K. ; Cullen, D. M. ; Akhter, Mohammed P. ; Haven, M. C. ; Schmid, M. / Effects of nicotine on bone and calciotropic hormones in growing female rats. In: Calcified Tissue International. 2000 ; Vol. 67, No. 1. pp. 68-74.
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