Effects of peripheral CCK receptor blockade on food intake in rats

Roger D. Reidelberger, Daniel A. Castellanos, Martin Hulce

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Type A cholecystokinin receptor (CCKAR) antagonists differing in blood-brain barrier permeability were used to test the hypothesis that satiety is mediated, in part, by CCK action at CCKARs located peripheral to the blood-brain barrier. At dark onset, non-food-deprived rats received a bolus injection of devazepide (2.5 μmol/kg iv), a 3-h infusion of A-70104 (1 or 3 μmol·kg -1·h -1 iv), or vehicle either alone or coadministered with a 3-h infusion of CCK-8 (10 nmol·kg -1·h -1 iv) or a 2-h intragastric infusion of peptone (1 g/h). Food intake was determined from continuous computer recordings of changes in food bowl weight. Devazepide penetrates the blood-brain barrier; A-70104, the dicyclohexylammonium salt of N α-3-quinolinoyl-D-Glu-N,N-dipentylamide (A-65186), does not. CCK-8 inhibited 3-h food intake by more than 50% and both A-70104 and devazepide abolished this response. A-70104 and devazepide stimulated food intake and similarly attenuated the anorexic response to intragastric infusion of peptone. Thus endogenous CCK appears to act, in part, at CCKARs peripheral to the blood-brain barrier to inhibit food intake.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume285
Issue number2 54-2
StatePublished - Aug 1 2003

Fingerprint

A 65186
Cholecystokinin Receptors
Devazepide
Blood-Brain Barrier
Eating
Sincalide
Peptones
Cholecystokinin A Receptor
Permeability
Salts
Weights and Measures
Food
Injections

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Effects of peripheral CCK receptor blockade on food intake in rats. / Reidelberger, Roger D.; Castellanos, Daniel A.; Hulce, Martin.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 285, No. 2 54-2, 01.08.2003.

Research output: Contribution to journalArticle

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