Abstract
Previous electrophoretic experiments suggest that the AP-1 site in duplex DNA bends in response to the pattern of amino acid charges distal to the basic region in bound bZIP proteins. The extent and direction of apparent DNA bending are consistent with the prediction that DNA will collapse locally upon asymmetric phosphate charge neutralization. To prove that asymmetric phosphate neutralization could produce the observed degree of DNA bending, the present experiments partially substitute anionic phosphate diesters in the AP-1 site with various numbers of neutral methylphosphonate linkages. DNA bending is induced toward the neutralized face of DNA. The degree of DNA bending induced by methylphosphonate substitution ( ~ 3.5°per neutralized phosphate) is comparable to that induced by GCN4 variants carrying increasing numbers of additional basic amino acids. It is plausible, therefore, that asymmetric phosphate neutralization is the cause of DNA bending in such complexes.
| Original language | English |
|---|---|
| Pages (from-to) | 2298-2305 |
| Number of pages | 8 |
| Journal | Nucleic Acids Research |
| Volume | 26 |
| Issue number | 10 |
| DOIs | |
| State | Published - May 15 1998 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Genetics
Cite this
Effects of phosphate neutralization on the shape of the AP-1 transcription factor binding site in duplex DNA. / Tomky, Laura A.; Strauss-Soukup, Juliane K.; Maher, L. James.
In: Nucleic Acids Research, Vol. 26, No. 10, 15.05.1998, p. 2298-2305.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of phosphate neutralization on the shape of the AP-1 transcription factor binding site in duplex DNA
AU - Tomky, Laura A.
AU - Strauss-Soukup, Juliane K.
AU - Maher, L. James
PY - 1998/5/15
Y1 - 1998/5/15
N2 - Previous electrophoretic experiments suggest that the AP-1 site in duplex DNA bends in response to the pattern of amino acid charges distal to the basic region in bound bZIP proteins. The extent and direction of apparent DNA bending are consistent with the prediction that DNA will collapse locally upon asymmetric phosphate charge neutralization. To prove that asymmetric phosphate neutralization could produce the observed degree of DNA bending, the present experiments partially substitute anionic phosphate diesters in the AP-1 site with various numbers of neutral methylphosphonate linkages. DNA bending is induced toward the neutralized face of DNA. The degree of DNA bending induced by methylphosphonate substitution ( ~ 3.5°per neutralized phosphate) is comparable to that induced by GCN4 variants carrying increasing numbers of additional basic amino acids. It is plausible, therefore, that asymmetric phosphate neutralization is the cause of DNA bending in such complexes.
AB - Previous electrophoretic experiments suggest that the AP-1 site in duplex DNA bends in response to the pattern of amino acid charges distal to the basic region in bound bZIP proteins. The extent and direction of apparent DNA bending are consistent with the prediction that DNA will collapse locally upon asymmetric phosphate charge neutralization. To prove that asymmetric phosphate neutralization could produce the observed degree of DNA bending, the present experiments partially substitute anionic phosphate diesters in the AP-1 site with various numbers of neutral methylphosphonate linkages. DNA bending is induced toward the neutralized face of DNA. The degree of DNA bending induced by methylphosphonate substitution ( ~ 3.5°per neutralized phosphate) is comparable to that induced by GCN4 variants carrying increasing numbers of additional basic amino acids. It is plausible, therefore, that asymmetric phosphate neutralization is the cause of DNA bending in such complexes.
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U2 - 10.1093/nar/26.10.2298
DO - 10.1093/nar/26.10.2298
M3 - Article
VL - 26
SP - 2298
EP - 2305
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 10
ER -