Effects of solution chemistry and aging time on prion protein adsorption and replication of soil-bound prions

Samuel E. Saunders, Qi Yuan, Jason C. Bartz, Shannon Bartelt-Hunt

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Prion interactions with soil may play an important role in the transmission of chronic wasting disease (CWD) and scrapie. Prions are known to bind to a wide range of soil surfaces, but the effects of adsorption solution chemistry and long-term soil binding on prion fate and transmission risk are unknown. We investigated HY TME prion protein (PrPSc) adsorption to soil minerals in aqueous solutions of phosphate buffered saline (PBS), sodium chloride, calcium chloride, and deionized water using western blotting. The replication efficiency of bound prions following adsorption in these solutions was also evaluated by protein misfolding cyclic amplification (PMCA). Aging studies investigated PrPSc desorption and replication efficiency up to one year following adsorption in PBS or DI water. Results indicate that adsorption solution chemistry can affect subsequent prion replication or desorption ability, especially after incubation periods of 30 d or longer. Observed effects were minor over the short-term (7 d or less). Results of long-term aging experiments demonstrate that unbound prions or prions bound to a diverse range of soil surfaces can readily replicate after one year. Our results suggest that while prion-soil interactions can vary with solution chemistry, prions bound to soil could remain a risk for transmitting prion diseases after months in the environment.

Original languageEnglish
Article numbere18752
JournalPLoS One
Volume6
Issue number4
DOIs
StatePublished - 2011

Fingerprint

Prions
prions
Adsorption
adsorption
chemistry
Soil
Aging of materials
Soils
soil
PrPSc proteins
desorption
Chronic Wasting Disease
Desorption
Phosphates
PrPSc Proteins
Prion Proteins
Scrapie
phosphates
Calcium Chloride
chronic wasting disease

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Effects of solution chemistry and aging time on prion protein adsorption and replication of soil-bound prions. / Saunders, Samuel E.; Yuan, Qi; Bartz, Jason C.; Bartelt-Hunt, Shannon.

In: PLoS One, Vol. 6, No. 4, e18752, 2011.

Research output: Contribution to journalArticle

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