Effects of trout endothelin on the motility of gastrointestinal smooth muscle from the trout and rat

Y. Wang, J. Jensen, Peter W. Abel, A. Fournier, S. Holmgren, J. M. Conlon

Research output: Contribution to journalArticle

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Abstract

Trout endothelin (ET), previously isolated from the kidney of the rainbow trout Oncorhynchus mykiss, contains four amino acid substitutions at residues 4-7 compared with rat ET-1. Trout ET produced sustained and concentration-dependent contractions of strips of longitudinal smooth muscle from trout stomach (pD2 = 7.52 ± 0.06) and proximal small intestine (pD2 = 7.80 ± 0.10) and from rat fundus (pD2 = 7.78 ± 0.14). Rat ET-1 was equipotent with trout ET for contraction of rat fundus and 2- to 3-fold more potent for contraction of trout gastrointestinal tissues. In contrast, rat ET-1 was 10- to 20-fold more potent than trout ET in constricting isolated rings of vascular tissue from trout efferent branchial artery and cardinal vein and from rat aorta (Y. Wang et al., 1999, Am. J. Physiol. 277, R1605-R1611). It is known that the contractile effects of ET-1 on rat fundus are mediated through the ETB receptor and effects on the rat aorta are mediated through the ETA receptor. We propose, therefore, that trout gastrointestinal tissues express an ETB-type receptor that differentiates poorly between trout ET and rat ET-1, whereas trout vascular tissues express an ETA-type receptor that is preferentially activated by rat ET-1. The rat ET-1-induced contractions of the trout gastrointestinal tissues are in part indirect, involving a serotoninergic neuronal pathway in the intestine and a noncholinergic, nonserotoninergic pathway in the stomach.

Original languageEnglish
Pages (from-to)156-162
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume123
Issue number2
DOIs
StatePublished - 2001

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gastrointestinal motility
endothelins
Gastrointestinal Motility
Trout
Endothelins
smooth muscle
trout
Smooth Muscle
Endothelin-1
rats
Oncorhynchus mykiss
receptors
vascular tissues
aorta
Blood Vessels
Aorta
Stomach
stomach
Amino Acid Substitution
amino acid substitution

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Effects of trout endothelin on the motility of gastrointestinal smooth muscle from the trout and rat. / Wang, Y.; Jensen, J.; Abel, Peter W.; Fournier, A.; Holmgren, S.; Conlon, J. M.

In: General and Comparative Endocrinology, Vol. 123, No. 2, 2001, p. 156-162.

Research output: Contribution to journalArticle

Wang, Y. ; Jensen, J. ; Abel, Peter W. ; Fournier, A. ; Holmgren, S. ; Conlon, J. M. / Effects of trout endothelin on the motility of gastrointestinal smooth muscle from the trout and rat. In: General and Comparative Endocrinology. 2001 ; Vol. 123, No. 2. pp. 156-162.
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