EGFR enhances early healing after cutaneous incisional wounding

Susan K. Repertinger, Erica Campagnaro, Jill Fuhrman, Taghrid El-Abaseri, Stuart H. Yuspa, Laura A. Hansen

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The epidermal growth factor receptor (EGFR) has been implicated in the regulation of wound healing. In order to directly evaluate the role of endogenous EGFR in cutaneous incisional wound healing, we examined EGFR null- and wild-type skin after injury. By 5 d after wounding, re-epithelialization was complete in all EGFR wild-type wounds, but in only 40% of EGFR null wounds. Delayed wound closure in EGFR null skin was accompanied by an increase in edema, longer lasting and more prominent eschar, and increased distance between apposing wound edges. EGFR altered neutrophil and mast cell infiltration, and enhanced angiogenesis. EGFR enhanced epithelial proliferation during the first 3 d following injury, although proliferation was greater in EGFR null wounds at 5 d. Although migration was decreased in EGFR null keratinocytes cultured with standard medium or in medium supplemented with transforming growth factor-α when compared with controls, the addition of the wound-associated motogen keratinocyte growth factor eliminated the differences between genotypes. Epithelial migration into the wound was decreased in EGFR null skin, suggesting that both EGFR-dependent and -independent mechanisms regulate migration during wound healing. These data demonstrate that EGFR regulates multiple facets of cutaneous wound healing, including inflammation, wound contraction, proliferation, migration, and angiogenesis.

Original languageEnglish
Pages (from-to)982-989
Number of pages8
JournalJournal of Investigative Dermatology
Volume123
Issue number5
DOIs
StatePublished - Nov 2004

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Epidermal Growth Factor Receptor
Skin
Wounds and Injuries
Wound Healing
Fibroblast Growth Factor 7
Re-Epithelialization
Transforming Growth Factors
Keratinocytes
Infiltration
Mast Cells
Edema
Neutrophils
Genotype
Inflammation

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

EGFR enhances early healing after cutaneous incisional wounding. / Repertinger, Susan K.; Campagnaro, Erica; Fuhrman, Jill; El-Abaseri, Taghrid; Yuspa, Stuart H.; Hansen, Laura A.

In: Journal of Investigative Dermatology, Vol. 123, No. 5, 11.2004, p. 982-989.

Research output: Contribution to journalArticle

Repertinger, SK, Campagnaro, E, Fuhrman, J, El-Abaseri, T, Yuspa, SH & Hansen, LA 2004, 'EGFR enhances early healing after cutaneous incisional wounding', Journal of Investigative Dermatology, vol. 123, no. 5, pp. 982-989. https://doi.org/10.1111/j.0022-202X.2004.23478.x
Repertinger, Susan K. ; Campagnaro, Erica ; Fuhrman, Jill ; El-Abaseri, Taghrid ; Yuspa, Stuart H. ; Hansen, Laura A. / EGFR enhances early healing after cutaneous incisional wounding. In: Journal of Investigative Dermatology. 2004 ; Vol. 123, No. 5. pp. 982-989.
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