Elevation of calcium in rat enterocytes by Escherichia coli heat-stable (STa) enterotoxin

Floyd C. Knoop, Michaela Owens, Joseph N. Marcus, Barbara Murphy

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The effect of STa enterotoxin on intracellular calcium in primary cultures of rat enterocytes was investigated. The enterocytes were loaded with a fluorescent calcium indicator, indo-1, and changes of intracellular calcium determined by flow cytometry. When the rat enterocytes were treated with EGTA, a calcium chelator, or ionomycin, a calcium ionophore, a decrease or increase, respectively, in free intracellular calcium was demonstrated. The addition of STa enterotoxin to rat enterocytes loaded with indo-1 caused an initial decrease, followed by a rapid increase, in free intracellular calcium. These effects on intracellular calcium occurred prior to or during an increase in the intracellular concentration of cyclic 3′,5′-guanosine monophosphate. The current results demonstrate that the activation of rat enterocytes by STa enterotoxin has an effect on transmembrane regulation of the receptor-dependent calcium signal and/or the release of calcium from intracellular storage sites.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalCurrent Microbiology
Volume23
Issue number5
DOIs
StatePublished - Nov 1 1991

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this