Emergence of carbapenem resistance due to the novel insertion sequence is Pa8 in pseudomonas aeruginosa

Randal C. Fowler, Nancy D. Hanson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chronic lung infections due to the persistence of Pseudomonas aeruginosa in cystic fibrosis patients are typically associated with the emergence of antibiotic resistance. The purpose of this study was to investigate the mechanisms responsible for the emergence of carbapenem resistance when a clinical isolate of P. aeruginosa collected from a patient with cystic fibrosis was challenged with meropenem. Nine carbapenem-resistant mutants were selected with subinhibitory concentrations of meropenem from a clinical isolate of P. aeruginosa and characterized for carbapenem resistance. Increased carbapenem MICs were associated with the identification of the novel insertion sequence ISPa8 within oprD or its promoter region in all the mutants. The position of ISPa8 was different for each of the mutants evaluated. In addition, Southern blot analyses identified multiple copies of ISPa8 within the genomes of the mutants and their parent isolate. These data demonstrate that transposition of IS elements within the Pseudomonas genome can influence antibiotic susceptibility. Understanding the selective pressures associated with the emergence of antibiotic resistance is critical for the judicious use of antimicrobial chemotherapy and the successful treatment of bacterial infections.

Original languageEnglish
Article numbere91299
JournalPLoS One
Volume9
Issue number3
DOIs
StatePublished - Apr 10 2014

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carbapenems
Carbapenems
Insertional Mutagenesis
meropenem
Pseudomonas aeruginosa
transposons
mutants
cystic fibrosis
Microbial Drug Resistance
Anti-Bacterial Agents
Cystic Fibrosis
antibiotic resistance
Genes
Genome
DNA Transposable Elements
Chemotherapy
genome
transposition (genetics)
Southern Blotting
Pseudomonas

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Emergence of carbapenem resistance due to the novel insertion sequence is Pa8 in pseudomonas aeruginosa. / Fowler, Randal C.; Hanson, Nancy D.

In: PLoS One, Vol. 9, No. 3, e91299, 10.04.2014.

Research output: Contribution to journalArticle

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