Abstract
Hydrogen sulfide (H 2S) is a novel gasotransmitter with physiological and pathological functions in vascular homeostasis, cardiovascular system and central nervous system. In the present study, we determined the endogenous levels of H 2S in various tissues of the bovine eye. We also examined the basal levels of H 2S in response to donors (sodium hydrosulfide, NaHS and sodium sulfide, Na 2S), substrate (l-cysteine), inhibitors (propargylglycine, PAG and aminooxyacetic acid, AOA) and activator (S-adenosyl-l-methionine, SAM) of this gas in the bovine retina. H 2S was measured using a well established spectrophotometric method. The highest concentration of endogenous H 2S was detected in cornea (19 ± 2.85 nmoles/mg protein, n = 6) and retina (17 ± 2.1 nmoles/mg protein, n = 6). Interestingly, H 2S was not present in vitreous humor. The inhibitors of CSE and CBS; PAG (1 mM) and AOA (1 mM), significantly attenuated the production of H 2S in the bovine retina by 56.8 and 42%, respectively. On the other hand the activator of CBS; SAM (100 μM), H 2S donors; NaHS (1 μM) and Na 2S (100 μM), significantly increased endogenous levels of H 2S in bovine retina. l-cysteine (10-300 μM) produced a significant (P <0.05) concentration-dependent increase in H 2S levels reaching a maximal at 300 μM. We conclude that H 2S is endogenously produced in various tissues of the isolated bovine eye. Moreover, endogenous levels of H 2S are enhanced in the presence of substrate (l-cysteine), an activator of CBS (SAM) and H 2S donors but are blocked by inhibitors of enzymes that synthesize this gas in neural retina.
| Original language | English |
|---|---|
| Pages (from-to) | 1540-1545 |
| Number of pages | 6 |
| Journal | Neurochemical Research |
| Volume | 36 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2011 |
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All Science Journal Classification (ASJC) codes
- Cellular and Molecular Neuroscience
- Biochemistry
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Endogenous production of hydrogen sulfide in isolated bovine eye. / Kulkarni, Madhura; Njie-Mbye, Ya Fatou; Okpobiri, Ikechukwu; Zhao, Min; Opere, Catherine A.; Ohia, Sunny E.
In: Neurochemical Research, Vol. 36, No. 8, 08.2011, p. 1540-1545.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endogenous production of hydrogen sulfide in isolated bovine eye
AU - Kulkarni, Madhura
AU - Njie-Mbye, Ya Fatou
AU - Okpobiri, Ikechukwu
AU - Zhao, Min
AU - Opere, Catherine A.
AU - Ohia, Sunny E.
PY - 2011/8
Y1 - 2011/8
N2 - Hydrogen sulfide (H 2S) is a novel gasotransmitter with physiological and pathological functions in vascular homeostasis, cardiovascular system and central nervous system. In the present study, we determined the endogenous levels of H 2S in various tissues of the bovine eye. We also examined the basal levels of H 2S in response to donors (sodium hydrosulfide, NaHS and sodium sulfide, Na 2S), substrate (l-cysteine), inhibitors (propargylglycine, PAG and aminooxyacetic acid, AOA) and activator (S-adenosyl-l-methionine, SAM) of this gas in the bovine retina. H 2S was measured using a well established spectrophotometric method. The highest concentration of endogenous H 2S was detected in cornea (19 ± 2.85 nmoles/mg protein, n = 6) and retina (17 ± 2.1 nmoles/mg protein, n = 6). Interestingly, H 2S was not present in vitreous humor. The inhibitors of CSE and CBS; PAG (1 mM) and AOA (1 mM), significantly attenuated the production of H 2S in the bovine retina by 56.8 and 42%, respectively. On the other hand the activator of CBS; SAM (100 μM), H 2S donors; NaHS (1 μM) and Na 2S (100 μM), significantly increased endogenous levels of H 2S in bovine retina. l-cysteine (10-300 μM) produced a significant (P <0.05) concentration-dependent increase in H 2S levels reaching a maximal at 300 μM. We conclude that H 2S is endogenously produced in various tissues of the isolated bovine eye. Moreover, endogenous levels of H 2S are enhanced in the presence of substrate (l-cysteine), an activator of CBS (SAM) and H 2S donors but are blocked by inhibitors of enzymes that synthesize this gas in neural retina.
AB - Hydrogen sulfide (H 2S) is a novel gasotransmitter with physiological and pathological functions in vascular homeostasis, cardiovascular system and central nervous system. In the present study, we determined the endogenous levels of H 2S in various tissues of the bovine eye. We also examined the basal levels of H 2S in response to donors (sodium hydrosulfide, NaHS and sodium sulfide, Na 2S), substrate (l-cysteine), inhibitors (propargylglycine, PAG and aminooxyacetic acid, AOA) and activator (S-adenosyl-l-methionine, SAM) of this gas in the bovine retina. H 2S was measured using a well established spectrophotometric method. The highest concentration of endogenous H 2S was detected in cornea (19 ± 2.85 nmoles/mg protein, n = 6) and retina (17 ± 2.1 nmoles/mg protein, n = 6). Interestingly, H 2S was not present in vitreous humor. The inhibitors of CSE and CBS; PAG (1 mM) and AOA (1 mM), significantly attenuated the production of H 2S in the bovine retina by 56.8 and 42%, respectively. On the other hand the activator of CBS; SAM (100 μM), H 2S donors; NaHS (1 μM) and Na 2S (100 μM), significantly increased endogenous levels of H 2S in bovine retina. l-cysteine (10-300 μM) produced a significant (P <0.05) concentration-dependent increase in H 2S levels reaching a maximal at 300 μM. We conclude that H 2S is endogenously produced in various tissues of the isolated bovine eye. Moreover, endogenous levels of H 2S are enhanced in the presence of substrate (l-cysteine), an activator of CBS (SAM) and H 2S donors but are blocked by inhibitors of enzymes that synthesize this gas in neural retina.
UR - http://www.scopus.com/inward/record.url?scp=79961211153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961211153&partnerID=8YFLogxK
U2 - 10.1007/s11064-011-0482-6
DO - 10.1007/s11064-011-0482-6
M3 - Article
C2 - 21533862
AN - SCOPUS:79961211153
VL - 36
SP - 1540
EP - 1545
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 8
ER -