Endogenous production of hydrogen sulfide in isolated bovine eye

Madhura Kulkarni, Ya Fatou Njie-Mbye, Ikechukwu Okpobiri, Min Zhao, Catherine A. Opere, Sunny E. Ohia

Research output: Contribution to journalArticle

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Abstract

Hydrogen sulfide (H 2S) is a novel gasotransmitter with physiological and pathological functions in vascular homeostasis, cardiovascular system and central nervous system. In the present study, we determined the endogenous levels of H 2S in various tissues of the bovine eye. We also examined the basal levels of H 2S in response to donors (sodium hydrosulfide, NaHS and sodium sulfide, Na 2S), substrate (l-cysteine), inhibitors (propargylglycine, PAG and aminooxyacetic acid, AOA) and activator (S-adenosyl-l-methionine, SAM) of this gas in the bovine retina. H 2S was measured using a well established spectrophotometric method. The highest concentration of endogenous H 2S was detected in cornea (19 ± 2.85 nmoles/mg protein, n = 6) and retina (17 ± 2.1 nmoles/mg protein, n = 6). Interestingly, H 2S was not present in vitreous humor. The inhibitors of CSE and CBS; PAG (1 mM) and AOA (1 mM), significantly attenuated the production of H 2S in the bovine retina by 56.8 and 42%, respectively. On the other hand the activator of CBS; SAM (100 μM), H 2S donors; NaHS (1 μM) and Na 2S (100 μM), significantly increased endogenous levels of H 2S in bovine retina. l-cysteine (10-300 μM) produced a significant (P <0.05) concentration-dependent increase in H 2S levels reaching a maximal at 300 μM. We conclude that H 2S is endogenously produced in various tissues of the isolated bovine eye. Moreover, endogenous levels of H 2S are enhanced in the presence of substrate (l-cysteine), an activator of CBS (SAM) and H 2S donors but are blocked by inhibitors of enzymes that synthesize this gas in neural retina.

Original languageEnglish
Pages (from-to)1540-1545
Number of pages6
JournalNeurochemical Research
Volume36
Issue number8
DOIs
StatePublished - Aug 2011

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Hydrogen Sulfide
Methionine
Cysteine
Retina
Gasotransmitters
Gases
Aminooxyacetic Acid
Tissue
Cardiovascular system
Neurology
Enzyme Inhibitors
Substrates
Proteins
Vitreous Body
Cardiovascular System
Cornea
Blood Vessels
Homeostasis
Central Nervous System
sodium bisulfide

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

Kulkarni, M., Njie-Mbye, Y. F., Okpobiri, I., Zhao, M., Opere, C. A., & Ohia, S. E. (2011). Endogenous production of hydrogen sulfide in isolated bovine eye. Neurochemical Research, 36(8), 1540-1545. https://doi.org/10.1007/s11064-011-0482-6

Endogenous production of hydrogen sulfide in isolated bovine eye. / Kulkarni, Madhura; Njie-Mbye, Ya Fatou; Okpobiri, Ikechukwu; Zhao, Min; Opere, Catherine A.; Ohia, Sunny E.

In: Neurochemical Research, Vol. 36, No. 8, 08.2011, p. 1540-1545.

Research output: Contribution to journalArticle

Kulkarni, M, Njie-Mbye, YF, Okpobiri, I, Zhao, M, Opere, CA & Ohia, SE 2011, 'Endogenous production of hydrogen sulfide in isolated bovine eye', Neurochemical Research, vol. 36, no. 8, pp. 1540-1545. https://doi.org/10.1007/s11064-011-0482-6
Kulkarni, Madhura ; Njie-Mbye, Ya Fatou ; Okpobiri, Ikechukwu ; Zhao, Min ; Opere, Catherine A. ; Ohia, Sunny E. / Endogenous production of hydrogen sulfide in isolated bovine eye. In: Neurochemical Research. 2011 ; Vol. 36, No. 8. pp. 1540-1545.
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