Endothelin-1 induces tyrosine phosphorylation in human blood monocytes

Lindsey J. Chisholm, Devendra K. Agrawal, Trevor J. Pearson, John D. Edwards

Research output: Contribution to journalArticle

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Abstract

Mediators including the neuropeptide endothelin-1 (ET-1), which are released in response to injury, modulate the expression of cell adhesion molecules on leukocytes and endothelial cells. The mechanisms underlying this process are not clear. In this study we investigated the effect of endothelin-1 on the expression of tyrosine phosphorylated proteins in human blood monocytes. Endothelin-1 caused an increase in tyrosine phosphorylated proteins in monocytes in a time-dependent and dose-dependent manner, the Mr 60, 80 and 110 kDa proteins being the most prominent. This effect was blocked by pre-incubating the monocytes with the selective tyrosine kinase inhibitors genistein or herbimycin A. Endothelin-1-induced upregulation of tyrosine phosphorylated proteins appears to be mediated by the ET(A) receptor. Unlike our previously reported studies in endothelial cells, immunoprecipitation with anti-src or anti-JAK antibodies followed by immunoblotting with PY20 in human blood monocytes revealed that these proteins of Mr 60, 80 and 110 kDa were not related to src or JAK kinases. These findings suggest that ET-1 exerts its effect on monocytes by a pathway involving tyrosine kinases other than src or JAK kinases.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume159
Issue number1
StatePublished - 1996

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Phosphorylation
Endothelin-1
Tyrosine
Monocytes
Blood
Janus Kinases
src-Family Kinases
Endothelial cells
Proteins
Protein-Tyrosine Kinases
Endothelial Cells
Genistein
Cell Adhesion Molecules
Neuropeptides
Immunoprecipitation
Immunoblotting
Anti-Idiotypic Antibodies
Leukocytes
Up-Regulation
Antibodies

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Clinical Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Endothelin-1 induces tyrosine phosphorylation in human blood monocytes. / Chisholm, Lindsey J.; Agrawal, Devendra K.; Pearson, Trevor J.; Edwards, John D.

In: Molecular and Cellular Biochemistry, Vol. 159, No. 1, 1996, p. 33-38.

Research output: Contribution to journalArticle

Chisholm, LJ, Agrawal, DK, Pearson, TJ & Edwards, JD 1996, 'Endothelin-1 induces tyrosine phosphorylation in human blood monocytes', Molecular and Cellular Biochemistry, vol. 159, no. 1, pp. 33-38.
Chisholm, Lindsey J. ; Agrawal, Devendra K. ; Pearson, Trevor J. ; Edwards, John D. / Endothelin-1 induces tyrosine phosphorylation in human blood monocytes. In: Molecular and Cellular Biochemistry. 1996 ; Vol. 159, No. 1. pp. 33-38.
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