Engineering precision RNA molecular switches

Garrett Soukup, Ronald R. Breaker

Research output: Contribution to journalArticle

263 Scopus citations

Abstract

Ligand-specific molecular switches composed of RNA were created by coupling preexisting catalytic and receptor domains via structural bridges. Binding of ligand to the receptor triggers a conformational change within the bridge, and this structural reorganization dictates the activity of the adjoining ribozyme. The modular nature of these tripartite constructs makes possible the rapid construction of precision RNA molecular switches that trigger only in the presence of their corresponding ligand. By using similar enzyme engineering strategies, new RNA switches can be made to operate as designer molecular sensors or as a new class of genetic control elements.

Original languageEnglish
Pages (from-to)3584-3589
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number7
DOIs
Publication statusPublished - Mar 30 1999

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All Science Journal Classification (ASJC) codes

  • General
  • Genetics

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