Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data

Anna C. Shore, Angela S. Rossney, Peter M. Kinnevey, Orla M. Brennan, Eilish Creamer, Orla Sherlock, Anthony Dolan, Robert Cunney, Derek J. Sullivan, Richard V. Goering, Hilary Humphreys, David C. Coleman

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

ST22-methicillin-resistant Staphylococcus aureus type IV (ST22-MRSA-IV) is endemic in Irish hospitals and is designated antibiogram-resistogram type-pulsed-field group (AR-PFG) 06-01. Isolates of this highly clonal strain exhibit limited numbers of pulsed-field gel electrophoresis (PFGE) patterns and spa types. This study investigated whether combining PFGE and spa typing with DNA sequencing of the staphylococcal cassette chromosome mec element (SCCmec)-associated direct repeat unit (dru typing) would improve isolate discrimination. A total of 173 MRSA isolates recovered in one Irish hospital during periods in 2007 and 2008 were investigated using antibiogram-resistogram (AR), PFGE, spa, dru, and SCCmec typing. Isolates representative of each of the 17 pulsed-field group 01 (PFG-01) spa types identified underwent multilocus sequence typing, and all isolates were ST22. Ninety-seven percent of isolates (168 of 173) exhibited AR-PFG 06-01 or closely related AR patterns, and 163 of these isolates harbored SCCmec type IVh. The combination of PFGE, spa, and dru typing methods significantly improved discrimination of the 168 PFG-01 isolates, yielding 65 type combinations with a Simpson's index of diversity (SID) of 96.53, compared to (i) pairwise combinations of spa and dru typing, spa and PFGE typing, and dru and PFGE typing, which yielded 37, 44, and 43 type combinations with SIDs of 90.84, 91.00, and 93.57, respectively, or (ii) individual spa, dru, and PFGE typing methods, which yielded 17, 17, and 21 types with SIDs of 66.9, 77.83, and 81.34, respectively. Analysis of epidemiological information for a subset of PFG-01 isolates validated the relationships inferred using combined PFGE, spa, and dru typing data. This approach significantly enhances discrimination of ST22-MRSA-IV isolates and could be applied to epidemiological investigations of other highly clonal MRSA strains.

Original languageEnglish
Pages (from-to)1839-1852
Number of pages14
JournalJournal of Clinical Microbiology
Volume48
Issue number5
DOIs
StatePublished - May 2010

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Pulsed Field Gel Electrophoresis
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Sudden Infant Death
Chromosomes
Multilocus Sequence Typing
homocysteine-thiolactone-nicotinamide
Nucleic Acid Repetitive Sequences
DNA Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data. / Shore, Anna C.; Rossney, Angela S.; Kinnevey, Peter M.; Brennan, Orla M.; Creamer, Eilish; Sherlock, Orla; Dolan, Anthony; Cunney, Robert; Sullivan, Derek J.; Goering, Richard V.; Humphreys, Hilary; Coleman, David C.

In: Journal of Clinical Microbiology, Vol. 48, No. 5, 05.2010, p. 1839-1852.

Research output: Contribution to journalArticle

Shore, AC, Rossney, AS, Kinnevey, PM, Brennan, OM, Creamer, E, Sherlock, O, Dolan, A, Cunney, R, Sullivan, DJ, Goering, RV, Humphreys, H & Coleman, DC 2010, 'Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data', Journal of Clinical Microbiology, vol. 48, no. 5, pp. 1839-1852. https://doi.org/10.1128/JCM.02155-09
Shore, Anna C. ; Rossney, Angela S. ; Kinnevey, Peter M. ; Brennan, Orla M. ; Creamer, Eilish ; Sherlock, Orla ; Dolan, Anthony ; Cunney, Robert ; Sullivan, Derek J. ; Goering, Richard V. ; Humphreys, Hilary ; Coleman, David C. / Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data. In: Journal of Clinical Microbiology. 2010 ; Vol. 48, No. 5. pp. 1839-1852.
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