Enhanced release of endothelium-derived relaxing factor in mineralocorticoid hypertension

Charles Bockman, W. B. Jeffries, W. A. Pettinger, Peter W. Abel

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Ring segments of superior mesenteric arteries studied in vitro were used to determine the role of the vascular endothelium in regulating vascular contractile and relaxant sensitivity in deoxycorticosterone acetate (DOCA)- salt hypertension. Wistar rats were given DOCA (20 mg/kg s.c. twice per week) and 1% NaCl drinking water for 5 weeks. In ring segments containing endothelium, there was a decrease in contractile sensitivity to arginine vasopressin, no change in contractile sensitivity to norepinephrine and KCl, and no change in relaxant sensitivity to acetylcholine or isoproterenol in arteries from hypertensive rats compared with normotensive controls. Removal of the vascular endothelium by rubbing had no effect on the contractile response to arginine vasopressin and KCl or the relaxant response to isoproterenol in control arteries. In arteries without endothelium, DOCA-salt hypertension caused a threefold increase in contractile sensitivity for arginine vasopressin, norepinephrine, and KCl; a 50% reduction in maximal relaxation to isoproterenol; and a threefold decrease in relaxant sensitivity to sodium nitroprusside. Indomethacin (10 μM) had no effect on contraction or relaxation. However, N-monomethyl L-arginine unmasked altered contractile sensitivity to norepinephrine in arteries from DOCA-salt hypertensive rats. These data show that the endothelium compensates for increased contractile and reduced relaxant responses of vascular muscle in DOCA-salt hypertension by increasing the release of endothelium-derived relaxing factor. These data suggest that altered vascular responsiveness is masked by the endothelium, thus preventing these alterations from contributing to increased peripheral resistance during the development of DOCA-salt hypertension.

Original languageEnglish
Pages (from-to)304-313
Number of pages10
JournalHypertension
Volume20
Issue number3
StatePublished - 1992

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Endothelium-Dependent Relaxing Factors
Desoxycorticosterone
Mineralocorticoids
Acetates
Hypertension
Salts
Endothelium
Arginine Vasopressin
Arteries
Isoproterenol
Blood Vessels
Norepinephrine
Vascular Endothelium
Superior Mesenteric Artery
Nitroprusside
Indomethacin
Drinking Water
Vascular Resistance
Acetylcholine
Arginine

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Enhanced release of endothelium-derived relaxing factor in mineralocorticoid hypertension. / Bockman, Charles; Jeffries, W. B.; Pettinger, W. A.; Abel, Peter W.

In: Hypertension, Vol. 20, No. 3, 1992, p. 304-313.

Research output: Contribution to journalArticle

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