Evidence for a similar receptor site for binding of [3H]leukotriene E4 and [3H]leukotriene D4 to the guinea-pig crude lung membrane

John B. Cheng; Robert G. Townley

doi:10.1016/0006-291X(84)91183-5

Evidence for a similar receptor site for binding of [³H]leukotriene E₄ and [³H]leukotriene D₄ to the guinea-pig crude lung membrane

John B. Cheng, Robert G. Townley

Department of Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

To determine whether the action of leukotriene E₄ is mediated by its cross reaction with leukotriene D₄ receptor sites, we compared [³H]leukotriene E₄ and [³H]leukotriene D₄ binding activities in selected tissues as well as their competition results in the guinea-pig crude lung membrane. We demonstrated good correlation of [³H]leukotriene E₄ and [³H]leukotriene D₄ binding activities among the tissues studied. A significant correlation was demonstrated between the ability of leukotriene C₄, D₄ and E₄, FPL-55712 and arachidonic acid to inhibit lung [³H]leukotriene E₄ and [³H]leukotriene D₄ binding. These correlations suggest that leukotriene E₄ binds to a site which is similar to or close to the leukotrine D₄ receptor.

Original language	English (US)
Pages (from-to)	949-954
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	122
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(84)91183-5
State	Published - Aug 16 1984

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Evidence for a similar receptor site for binding of [3H]leukotriene E4 and [3H]leukotriene D4 to the guinea-pig crude lung membrane",

abstract = "To determine whether the action of leukotriene E4 is mediated by its cross reaction with leukotriene D4 receptor sites, we compared [3H]leukotriene E4 and [3H]leukotriene D4 binding activities in selected tissues as well as their competition results in the guinea-pig crude lung membrane. We demonstrated good correlation of [3H]leukotriene E4 and [3H]leukotriene D4 binding activities among the tissues studied. A significant correlation was demonstrated between the ability of leukotriene C4, D4 and E4, FPL-55712 and arachidonic acid to inhibit lung [3H]leukotriene E4 and [3H]leukotriene D4 binding. These correlations suggest that leukotriene E4 binds to a site which is similar to or close to the leukotrine D4 receptor.",

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N2 - To determine whether the action of leukotriene E4 is mediated by its cross reaction with leukotriene D4 receptor sites, we compared [3H]leukotriene E4 and [3H]leukotriene D4 binding activities in selected tissues as well as their competition results in the guinea-pig crude lung membrane. We demonstrated good correlation of [3H]leukotriene E4 and [3H]leukotriene D4 binding activities among the tissues studied. A significant correlation was demonstrated between the ability of leukotriene C4, D4 and E4, FPL-55712 and arachidonic acid to inhibit lung [3H]leukotriene E4 and [3H]leukotriene D4 binding. These correlations suggest that leukotriene E4 binds to a site which is similar to or close to the leukotrine D4 receptor.

AB - To determine whether the action of leukotriene E4 is mediated by its cross reaction with leukotriene D4 receptor sites, we compared [3H]leukotriene E4 and [3H]leukotriene D4 binding activities in selected tissues as well as their competition results in the guinea-pig crude lung membrane. We demonstrated good correlation of [3H]leukotriene E4 and [3H]leukotriene D4 binding activities among the tissues studied. A significant correlation was demonstrated between the ability of leukotriene C4, D4 and E4, FPL-55712 and arachidonic acid to inhibit lung [3H]leukotriene E4 and [3H]leukotriene D4 binding. These correlations suggest that leukotriene E4 binds to a site which is similar to or close to the leukotrine D4 receptor.

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Evidence for a similar receptor site for binding of [³H]leukotriene E₄ and [³H]leukotriene D₄ to the guinea-pig crude lung membrane

Abstract

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