Expanding the described metabolome of the marine cyanobacterium moorea producens jhb through orthogonal natural products workflows

Paul D. Boudreau, Emily A. Monroe, Suneet Mehrotra, Shane Desfor, Anton Korobeynikov, David H. Sherman, Thomas F. Murray, Lena Gerwick, Pieter C. Dorrestein, William H. Gerwick

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15 Scopus citations

Abstract

Moorea producens JHB, a Jamaican strain of tropical filamentous marine cyanobacteria, has been extensively studied by traditional natural products techniques. These previous bioassay and structure guided isolations led to the discovery of two exciting classes of natural products, hectochlorin (1) and jamaicamides A (2) and B (3). In the current study, mass spectrometry-based 'molecular networking' was used to visualize the metabolome of Moorea producens JHB, and both guided and enhanced the isolation workflow, revealing additional metabolites in these compound classes. Further, we developed additional insight into the metabolic capabilities of this strain by genome sequencing analysis, which subsequently led to the isolation of a compound unrelated to the jamaicamide and hectochlorin families. Another approach involved stimulation of the biosynthesis of a minor jamaicamide metabolite by cultivation in modified media, and provided insights about the underlying biosynthetic machinery as well as preliminary structure-Activity information within this structure class. This study demonstrated that these orthogonal approaches are complementary and enrich secondary metabolomic coverage even in an extensively studied bacterial strain.

Original languageEnglish (US)
Article numbere0133297
JournalPloS one
Volume10
Issue number7
DOIs
StatePublished - Jul 29 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Boudreau, P. D., Monroe, E. A., Mehrotra, S., Desfor, S., Korobeynikov, A., Sherman, D. H., Murray, T. F., Gerwick, L., Dorrestein, P. C., & Gerwick, W. H. (2015). Expanding the described metabolome of the marine cyanobacterium moorea producens jhb through orthogonal natural products workflows. PloS one, 10(7), [e0133297]. https://doi.org/10.1371/journal.pone.0133297