Abstract
An in vivo animal model was used to assess the enteropathogenicity of the etiological agent (Treponema hyodysenteriae) of swine dysentery. Multiple ligated ileal loops, prepared in New Zealand white rabbits, were challenged with either pathogenic (B78 and B204) or nonpathogenic (Pu) isolates of the organism. The pathogenic isolates induced the onset of intestinal fluid accumulation as early as 4 hr, with maximal fluid induction at 18 hr postchallenge. Gross lesions of the intestinal mucosa, observed in ileal loops of rabbits sacrificed 24 hr postchallenge, were characteristic of swine dysentery. Both pathogenic isolates colonized the epithelial surface and eroded the mucosal barrier, as determined by histological and scanning electron microscopic observations. Intestinal fluid accumulation and erosion of the mucosal barrier were not observed in ileal loops exposed to the nonpathogenic isolate (Pu) or to either of the nonviable pathogenic (B78 and B204) isolates. The ability of pathogenic isolates to initiate and produce infection in rabbit ligated ileal loops, which closely resembles the disease in swine, provides a system with which to study experimental swine dysentery.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1196-1201 |
| Number of pages | 6 |
| Journal | Infection and Immunity |
| Volume | 26 |
| Issue number | 3 |
| State | Published - Dec 1 1979 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Immunology
Cite this
Experimental infection of rabbit ligated ileal loops with Treponema hyodysenteriae. / Knoop, Floyd C.
In: Infection and Immunity, Vol. 26, No. 3, 01.12.1979, p. 1196-1201.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Experimental infection of rabbit ligated ileal loops with Treponema hyodysenteriae
AU - Knoop, Floyd C.
PY - 1979/12/1
Y1 - 1979/12/1
N2 - An in vivo animal model was used to assess the enteropathogenicity of the etiological agent (Treponema hyodysenteriae) of swine dysentery. Multiple ligated ileal loops, prepared in New Zealand white rabbits, were challenged with either pathogenic (B78 and B204) or nonpathogenic (Pu) isolates of the organism. The pathogenic isolates induced the onset of intestinal fluid accumulation as early as 4 hr, with maximal fluid induction at 18 hr postchallenge. Gross lesions of the intestinal mucosa, observed in ileal loops of rabbits sacrificed 24 hr postchallenge, were characteristic of swine dysentery. Both pathogenic isolates colonized the epithelial surface and eroded the mucosal barrier, as determined by histological and scanning electron microscopic observations. Intestinal fluid accumulation and erosion of the mucosal barrier were not observed in ileal loops exposed to the nonpathogenic isolate (Pu) or to either of the nonviable pathogenic (B78 and B204) isolates. The ability of pathogenic isolates to initiate and produce infection in rabbit ligated ileal loops, which closely resembles the disease in swine, provides a system with which to study experimental swine dysentery.
AB - An in vivo animal model was used to assess the enteropathogenicity of the etiological agent (Treponema hyodysenteriae) of swine dysentery. Multiple ligated ileal loops, prepared in New Zealand white rabbits, were challenged with either pathogenic (B78 and B204) or nonpathogenic (Pu) isolates of the organism. The pathogenic isolates induced the onset of intestinal fluid accumulation as early as 4 hr, with maximal fluid induction at 18 hr postchallenge. Gross lesions of the intestinal mucosa, observed in ileal loops of rabbits sacrificed 24 hr postchallenge, were characteristic of swine dysentery. Both pathogenic isolates colonized the epithelial surface and eroded the mucosal barrier, as determined by histological and scanning electron microscopic observations. Intestinal fluid accumulation and erosion of the mucosal barrier were not observed in ileal loops exposed to the nonpathogenic isolate (Pu) or to either of the nonviable pathogenic (B78 and B204) isolates. The ability of pathogenic isolates to initiate and produce infection in rabbit ligated ileal loops, which closely resembles the disease in swine, provides a system with which to study experimental swine dysentery.
UR - http://www.scopus.com/inward/record.url?scp=0018725794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018725794&partnerID=8YFLogxK
M3 - Article
VL - 26
SP - 1196
EP - 1201
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 3
ER -