Expression, functional, and structural analysis of proteins critical for otoconia development

Yinfang Xu, Hui Zhang, Hua Yang, Xing Zhao, Sándor Lovas, Yunxia Yesha Wang Lundberg

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Otoconia, developed during late gestation and perinatal stages, couple mechanic force to the sensory hair cells in the vestibule for motion detection and bodily balance. In the present work, we have investigated whether compensatory deposition of another protein(s) may have taken place to partially alleviate the detrimental effects of Oc90 deletion by analyzing a comprehensive list of plausible candidates, and have found a drastic increase in the deposition of Sparc-like 1 (aka Sc1 or hevin) in Oc90 null versus wt otoconia. We show that such up-regulation is specific to Sc1, and that stable transfection of Oc90 and Sc1 fulllength expression constructs in NIH/3T3 cells indeed promotes matrix calcification. Analysis and modeling of Oc90 and Sc1 protein structures show common features that may be critical requirements for the otoconial matrix backbone protein. Such information will serve as the foundation for future regenerative purposes.

Original languageEnglish (US)
Pages (from-to)2659-2673
Number of pages15
JournalDevelopmental Dynamics
Volume239
Issue number10
DOIs
StatePublished - Oct 2010

All Science Journal Classification (ASJC) codes

  • Developmental Biology

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