Expression of adenylyl cyclase type I in cochlear inner hair cells

Marian J. Drescher, Khalid M. Khan, Kirk Beisel, Ahmad A. Karadaghy, James S. Hatfield, Steve Y. Kim, Andrew J. Drescher, John M. Lasak, Roberto L. Barretto, Ali H. Shakir, Dennis G. Drescher

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Expression of calcium/calmodulin-activated adenylyl cyclase type I (ACI) mRNA has been determined in the cochlea and in an organ-of-Corti subdissected tissue fraction by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Amplification products of predicted size were obtained from the mouse cochlea and rat organ of Corti with nucleotide sequences corresponding to respective ACI brain transcripts. In addition, ACI template was detected in a rat inner hair cell cDNA library by PCR. Immunoreactivity to ACI has been localized within the organ of Corti to the inner hair cell, with diaminobenzidine staining found in both the cell body and in the stereocilia. Evidence, thus, has been obtained that both ACI transcript and protein are expressed in the inner hair cell, the primary mechanosensory receptor cell of the cochlea. We hypothesize that ACI is activated by calcium influx through a calcium/calmodulin interaction and that this adenylyl cyclase isoform may have a role in modulation of receptoneural afferent transmission and/or mechanosensory transduction in the cochlea.

Original languageEnglish
Pages (from-to)325-330
Number of pages6
JournalMolecular Brain Research
Volume45
Issue number2
DOIs
StatePublished - May 1997

Fingerprint

Inner Auditory Hair Cells
Cochlea
Organ of Corti
Calmodulin
Calcium
Cellular Mechanotransduction
Stereocilia
Reverse Transcriptase Polymerase Chain Reaction
Gene Library
Adenylyl Cyclases
adenylyl cyclase 1
Protein Isoforms
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Drescher, M. J., Khan, K. M., Beisel, K., Karadaghy, A. A., Hatfield, J. S., Kim, S. Y., ... Drescher, D. G. (1997). Expression of adenylyl cyclase type I in cochlear inner hair cells. Molecular Brain Research, 45(2), 325-330. https://doi.org/10.1016/S0169-328X(97)00007-7

Expression of adenylyl cyclase type I in cochlear inner hair cells. / Drescher, Marian J.; Khan, Khalid M.; Beisel, Kirk; Karadaghy, Ahmad A.; Hatfield, James S.; Kim, Steve Y.; Drescher, Andrew J.; Lasak, John M.; Barretto, Roberto L.; Shakir, Ali H.; Drescher, Dennis G.

In: Molecular Brain Research, Vol. 45, No. 2, 05.1997, p. 325-330.

Research output: Contribution to journalArticle

Drescher, MJ, Khan, KM, Beisel, K, Karadaghy, AA, Hatfield, JS, Kim, SY, Drescher, AJ, Lasak, JM, Barretto, RL, Shakir, AH & Drescher, DG 1997, 'Expression of adenylyl cyclase type I in cochlear inner hair cells', Molecular Brain Research, vol. 45, no. 2, pp. 325-330. https://doi.org/10.1016/S0169-328X(97)00007-7
Drescher, Marian J. ; Khan, Khalid M. ; Beisel, Kirk ; Karadaghy, Ahmad A. ; Hatfield, James S. ; Kim, Steve Y. ; Drescher, Andrew J. ; Lasak, John M. ; Barretto, Roberto L. ; Shakir, Ali H. ; Drescher, Dennis G. / Expression of adenylyl cyclase type I in cochlear inner hair cells. In: Molecular Brain Research. 1997 ; Vol. 45, No. 2. pp. 325-330.
@article{0e9e4bbf39004439bf79f8e0f5c4879e,
title = "Expression of adenylyl cyclase type I in cochlear inner hair cells",
abstract = "Expression of calcium/calmodulin-activated adenylyl cyclase type I (ACI) mRNA has been determined in the cochlea and in an organ-of-Corti subdissected tissue fraction by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Amplification products of predicted size were obtained from the mouse cochlea and rat organ of Corti with nucleotide sequences corresponding to respective ACI brain transcripts. In addition, ACI template was detected in a rat inner hair cell cDNA library by PCR. Immunoreactivity to ACI has been localized within the organ of Corti to the inner hair cell, with diaminobenzidine staining found in both the cell body and in the stereocilia. Evidence, thus, has been obtained that both ACI transcript and protein are expressed in the inner hair cell, the primary mechanosensory receptor cell of the cochlea. We hypothesize that ACI is activated by calcium influx through a calcium/calmodulin interaction and that this adenylyl cyclase isoform may have a role in modulation of receptoneural afferent transmission and/or mechanosensory transduction in the cochlea.",
author = "Drescher, {Marian J.} and Khan, {Khalid M.} and Kirk Beisel and Karadaghy, {Ahmad A.} and Hatfield, {James S.} and Kim, {Steve Y.} and Drescher, {Andrew J.} and Lasak, {John M.} and Barretto, {Roberto L.} and Shakir, {Ali H.} and Drescher, {Dennis G.}",
year = "1997",
month = "5",
doi = "10.1016/S0169-328X(97)00007-7",
language = "English",
volume = "45",
pages = "325--330",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Expression of adenylyl cyclase type I in cochlear inner hair cells

AU - Drescher, Marian J.

AU - Khan, Khalid M.

AU - Beisel, Kirk

AU - Karadaghy, Ahmad A.

AU - Hatfield, James S.

AU - Kim, Steve Y.

AU - Drescher, Andrew J.

AU - Lasak, John M.

AU - Barretto, Roberto L.

AU - Shakir, Ali H.

AU - Drescher, Dennis G.

PY - 1997/5

Y1 - 1997/5

N2 - Expression of calcium/calmodulin-activated adenylyl cyclase type I (ACI) mRNA has been determined in the cochlea and in an organ-of-Corti subdissected tissue fraction by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Amplification products of predicted size were obtained from the mouse cochlea and rat organ of Corti with nucleotide sequences corresponding to respective ACI brain transcripts. In addition, ACI template was detected in a rat inner hair cell cDNA library by PCR. Immunoreactivity to ACI has been localized within the organ of Corti to the inner hair cell, with diaminobenzidine staining found in both the cell body and in the stereocilia. Evidence, thus, has been obtained that both ACI transcript and protein are expressed in the inner hair cell, the primary mechanosensory receptor cell of the cochlea. We hypothesize that ACI is activated by calcium influx through a calcium/calmodulin interaction and that this adenylyl cyclase isoform may have a role in modulation of receptoneural afferent transmission and/or mechanosensory transduction in the cochlea.

AB - Expression of calcium/calmodulin-activated adenylyl cyclase type I (ACI) mRNA has been determined in the cochlea and in an organ-of-Corti subdissected tissue fraction by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Amplification products of predicted size were obtained from the mouse cochlea and rat organ of Corti with nucleotide sequences corresponding to respective ACI brain transcripts. In addition, ACI template was detected in a rat inner hair cell cDNA library by PCR. Immunoreactivity to ACI has been localized within the organ of Corti to the inner hair cell, with diaminobenzidine staining found in both the cell body and in the stereocilia. Evidence, thus, has been obtained that both ACI transcript and protein are expressed in the inner hair cell, the primary mechanosensory receptor cell of the cochlea. We hypothesize that ACI is activated by calcium influx through a calcium/calmodulin interaction and that this adenylyl cyclase isoform may have a role in modulation of receptoneural afferent transmission and/or mechanosensory transduction in the cochlea.

UR - http://www.scopus.com/inward/record.url?scp=0031149107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031149107&partnerID=8YFLogxK

U2 - 10.1016/S0169-328X(97)00007-7

DO - 10.1016/S0169-328X(97)00007-7

M3 - Article

VL - 45

SP - 325

EP - 330

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -