Expression of adenylyl cyclase type I in cochlear inner hair cells

Marian J. Drescher, Khalid M. Khan, Kirk W. Beisel, Ahmad A. Karadaghy, James S. Hatfield, Steve Y. Kim, Andrew J. Drescher, John M. Lasak, Roberto L. Barretto, Ali H. Shakir, Dennis G. Drescher

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Expression of calcium/calmodulin-activated adenylyl cyclase type I (ACI) mRNA has been determined in the cochlea and in an organ-of-Corti subdissected tissue fraction by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Amplification products of predicted size were obtained from the mouse cochlea and rat organ of Corti with nucleotide sequences corresponding to respective ACI brain transcripts. In addition, ACI template was detected in a rat inner hair cell cDNA library by PCR. Immunoreactivity to ACI has been localized within the organ of Corti to the inner hair cell, with diaminobenzidine staining found in both the cell body and in the stereocilia. Evidence, thus, has been obtained that both ACI transcript and protein are expressed in the inner hair cell, the primary mechanosensory receptor cell of the cochlea. We hypothesize that ACI is activated by calcium influx through a calcium/calmodulin interaction and that this adenylyl cyclase isoform may have a role in modulation of receptoneural afferent transmission and/or mechanosensory transduction in the cochlea.

Original languageEnglish (US)
Pages (from-to)325-330
Number of pages6
JournalMolecular Brain Research
Issue number2
StatePublished - May 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Expression of adenylyl cyclase type I in cochlear inner hair cells'. Together they form a unique fingerprint.

Cite this