Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii

Hiroe Suzuki, Shawichi Iwamuro, Aya Ohnuma, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, Hubert Vaudry, Christopher K. Taylor, Peter W. Abel, J. Michael Conlon

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Peptidomic analysis of an extract of the skin of the stream brown frog Rana sakuraii Matsui and Matsui, 1990 led to the isolation of a C-terminally α-amidated peptide (VR-23; VIGSILGALASGLPTLISWIKNR·NH2) with broad-spectrum antimicrobial activity that shows structural similarity to the bee venom peptide, melittin together with two peptides belonging to the temporin family (temporin-1SKa; FLPVILPVIGKLLNGIL·NH2 and temporin-1SKb; FLPVILPVIGKLLSGIL·NH2), and peptides whose primary structures identified them as belonging to the brevinin-2 (2 peptides) and ranatuerin-2 (1 peptide) families. Using a forward primer that was designed from a conserved region of the 5′-untranslated regions of Rana temporaria preprotemporins in a 3′-RACE procedure, a cDNA clone encoding preprotemporin-1SKa was prepared from R. sakuraii skin total RNA. Further preprotemporin cDNAs encoding temporin-1SKc (AVDLAKIANIAN KVLSSL F·NH2) and temporin-1SKd (FLPMLAKLLSGFL·NH2) were obtained by RT-PCR. Unexpectedly, the 3′-RACE procedure using the same primer led to amplification of a cDNA encoding a preprobradykinin whose signal peptide region was identical to that of preprotemporin-1SKa except for the substitution Ser18 → Asn. R. sakuraii bradykinin ([Arg0,Leu1,Thr6,Trp8] BK) was 28-fold less potent than mammalian BK in effecting B2 receptor-mediated relaxation of mouse trachea and the des[Arg0] derivative was only a weak partial agonist. The evolutionary history of the Japanese brown frogs is incompletely understood but a comparison of the primary structures of the R. sakuraii dermal peptides with those of Tago's brown frog Rana tagoi provides evidence for a close phylogenetic relationship between these species.

Original languageEnglish
Pages (from-to)505-514
Number of pages10
JournalPeptides
Volume28
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Ranidae
Gene encoding
Bradykinin
Anura
Skin
Gene Expression
Peptides
Complementary DNA
Rana temporaria
Melitten
Bee Venoms
5' Untranslated Regions
Protein Sorting Signals
Trachea
Amplification
Substitution reactions
Clone Cells
History
temporin
RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Suzuki, H., Iwamuro, S., Ohnuma, A., Coquet, L., Leprince, J., Jouenne, T., ... Conlon, J. M. (2007). Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii. Peptides, 28(3), 505-514. https://doi.org/10.1016/j.peptides.2006.10.016

Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii. / Suzuki, Hiroe; Iwamuro, Shawichi; Ohnuma, Aya; Coquet, Laurent; Leprince, Jérôme; Jouenne, Thierry; Vaudry, Hubert; Taylor, Christopher K.; Abel, Peter W.; Conlon, J. Michael.

In: Peptides, Vol. 28, No. 3, 03.2007, p. 505-514.

Research output: Contribution to journalArticle

Suzuki, H, Iwamuro, S, Ohnuma, A, Coquet, L, Leprince, J, Jouenne, T, Vaudry, H, Taylor, CK, Abel, PW & Conlon, JM 2007, 'Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii', Peptides, vol. 28, no. 3, pp. 505-514. https://doi.org/10.1016/j.peptides.2006.10.016
Suzuki, Hiroe ; Iwamuro, Shawichi ; Ohnuma, Aya ; Coquet, Laurent ; Leprince, Jérôme ; Jouenne, Thierry ; Vaudry, Hubert ; Taylor, Christopher K. ; Abel, Peter W. ; Conlon, J. Michael. / Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii. In: Peptides. 2007 ; Vol. 28, No. 3. pp. 505-514.
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AU - Leprince, Jérôme

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