Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon

Shantibhusan Senapati; Samuel B. Ho; Poonam Sharma; Srustidhar Das; Subhankar Chakraborty; Sukhwinder Kaur; Gloria Niehans; Surinder K. Batra

doi:10.1136/jcp.2010.078717

Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon

Shantibhusan Senapati, Samuel B. Ho, Poonam Sharma, Srustidhar Das, Subhankar Chakraborty, Sukhwinder Kaur, Gloria Niehans, Surinder K. Batra

Department of Pathology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p

Original language	English (US)
Pages (from-to)	702-707
Number of pages	6
Journal	Journal of Clinical Pathology
Volume	63
Issue number	8
DOIs	https://doi.org/10.1136/jcp.2010.078717
State	Published - Aug 2010

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine

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10.1136/jcp.2010.078717

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title = "Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon",

abstract = "Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p",

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AU - Senapati, Shantibhusan

AU - Ho, Samuel B.

AU - Sharma, Poonam

AU - Das, Srustidhar

AU - Chakraborty, Subhankar

AU - Kaur, Sukhwinder

AU - Niehans, Gloria

AU - Batra, Surinder K.

PY - 2010/8

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N2 - Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p

AB - Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p

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Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon

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