Abstract
Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p
| Original language | English |
|---|---|
| Pages (from-to) | 702-707 |
| Number of pages | 6 |
| Journal | Journal of Clinical Pathology - Clinical Molecular Pathology |
| Volume | 63 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2010 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
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Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon. / Senapati, Shantibhusan; Ho, Samuel B.; Sharma, Poonam K; Das, Srustidhar; Chakraborty, Subhankar; Kaur, Sukhwinder; Niehans, Gloria; Batra, Surinder K.
In: Journal of Clinical Pathology - Clinical Molecular Pathology, Vol. 63, No. 8, 08.2010, p. 702-707.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon
AU - Senapati, Shantibhusan
AU - Ho, Samuel B.
AU - Sharma, Poonam K
AU - Das, Srustidhar
AU - Chakraborty, Subhankar
AU - Kaur, Sukhwinder
AU - Niehans, Gloria
AU - Batra, Surinder K.
PY - 2010/8
Y1 - 2010/8
N2 - Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p
AB - Aim: To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon. Methods: Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR. Results: MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p
UR - http://www.scopus.com/inward/record.url?scp=77955722731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955722731&partnerID=8YFLogxK
U2 - 10.1136/jcp.2010.078717
DO - 10.1136/jcp.2010.078717
M3 - Article
C2 - 20702471
AN - SCOPUS:77955722731
VL - 63
SP - 702
EP - 707
JO - Journal of Clinical Pathology
JF - Journal of Clinical Pathology
SN - 0021-9746
IS - 8
ER -