TY - JOUR
T1 - Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood
AU - Clinton, Sarah M.
AU - Glover, Matthew E.
AU - Maltare, Astha
AU - Laszczyk, Ann M.
AU - Mehi, Stephen J.
AU - Simmons, Rebecca K.
AU - King, Gwendalyn D.
N1 - Funding Information:
We are grateful to Sharon Burke and the laboratory of Dr. Stanley Watson at the University of Michigan for designing and providing the subclone for the kl riboprobe. We are also grateful to Terry Lewis and the UAB Molecular Detection Core for vital help in developing the immunohistochemistry protocol (P30 NS047466). We thank the Alabama Neuroscience Blueprint Core Center (P30-NS57098), the UAB Intellectual and Developmental Disabilities Research Center (P30-HD38985), and the UAB Neuroscience Core (P30-NS47466) for instrumentation. This study was supported by NIH/NIMH R00 MH085859-03 (SMC) and NIH/NIA R00 AG034989 (GDK).
PY - 2013/8/21
Y1 - 2013/8/21
N2 - Without the age-regulating protein klotho, mouse lifespan is shortened and the rapid onset of age-related disorders occurs. Conversely, overexpression of klotho extends mouse lifespan. Klotho is most abundant in kidney and expressed in a limited number of other organs, including the brain, where klotho levels are highest in choroid plexus. Reports vary on where klotho is expressed within the brain parenchyma, and no data is available as to whether klotho levels change across postnatal development. We used in situ hybridization to map klotho mRNA expression in the developing and adult rat brain and report moderate, widespread expression across grey matter regions. mRNA expression levels in cortex, hippocampus, caudate putamen, and amygdala decreased during the second week of life and then gradually rose to adult levels by postnatal day 21. Immunohistochemistry revealed a protein expression pattern similar to the mRNA results, with klotho protein expressed widely throughout the brain. Klotho protein co-localized with both the neuronal marker NeuN, as well as, oligodendrocyte marker olig2. These results provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development.
AB - Without the age-regulating protein klotho, mouse lifespan is shortened and the rapid onset of age-related disorders occurs. Conversely, overexpression of klotho extends mouse lifespan. Klotho is most abundant in kidney and expressed in a limited number of other organs, including the brain, where klotho levels are highest in choroid plexus. Reports vary on where klotho is expressed within the brain parenchyma, and no data is available as to whether klotho levels change across postnatal development. We used in situ hybridization to map klotho mRNA expression in the developing and adult rat brain and report moderate, widespread expression across grey matter regions. mRNA expression levels in cortex, hippocampus, caudate putamen, and amygdala decreased during the second week of life and then gradually rose to adult levels by postnatal day 21. Immunohistochemistry revealed a protein expression pattern similar to the mRNA results, with klotho protein expressed widely throughout the brain. Klotho protein co-localized with both the neuronal marker NeuN, as well as, oligodendrocyte marker olig2. These results provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development.
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U2 - 10.1016/j.brainres.2013.06.044
DO - 10.1016/j.brainres.2013.06.044
M3 - Article
C2 - 23838326
AN - SCOPUS:84881146647
VL - 1527
SP - 1
EP - 14
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -