FGF9 can induce endochondral ossification in cranial mesenchyme

Venkatesh Govindarajan, Paul A. Overbeek

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: The flat bones of the skull (i.e., the frontal and parietal bones) normally form through intramembranous ossification. At these sites cranial mesenchymal cells directly differentiate into osteoblasts without the formation of a cartilage intermediate. This type of ossification is distinct from endochondral ossification, a process that involves initial formation of cartilage and later replacement by bone. Results: We have analyzed a line of transgenic mice that expresses FGF9, a member of the fibroblast growth factor family (FGF), in cranial mesenchymal cells. The parietal bones in these mice show a switch from intramembranous to endochondral ossification. Cranial cartilage precursors are induced to proliferate, then hypertrophy and are later replaced by bone. These changes are accompanied by upregulation of Sox9, Ihh, Col2a1, Col10a1 and downregulation of Cbfal and Osteocalcin. Fate mapping studies show that the cranial mesenchymal cells in the parietal region that show a switch in cell fate are likely to be derived from the mesoderm. Conclusion: These results demonstrate that FGF9 expression is sufficient to convert the differentiation program of (at least a subset of) mesoderm-derived cranial mesenchyme cells from intramembranous to endochondral ossification.

Original languageEnglish
Article number7
JournalBMC Developmental Biology
Volume6
DOIs
StatePublished - Feb 20 2006

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Mesoderm
Osteogenesis
Parietal Bone
Cartilage
Bone and Bones
Frontal Bone
Parietal Lobe
Fibroblast Growth Factors
Osteocalcin
Osteoblasts
Skull
Hypertrophy
Transgenic Mice
Up-Regulation
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

FGF9 can induce endochondral ossification in cranial mesenchyme. / Govindarajan, Venkatesh; Overbeek, Paul A.

In: BMC Developmental Biology, Vol. 6, 7, 20.02.2006.

Research output: Contribution to journalArticle

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