Fingerstick glycosylated hemoglobin, plasma protein, and albumin

M. Rendell, C. Brannan, J. Nierenberg, K. Rasbold, T. Hestorff

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have developed techniques that permit the affinity-chromatographic determination of glycosylated hemoglobin, plasma protein, and albumin on fingerstick samples of whole blood. The fingerstick glycohemoglobin technique takes advantage of the high sensitivity of measurement of hemoglobin by absorbance at 414 nm. The glycosylated plasma protein is assayed by a highly sensitive method based on binding of Coomassie blue. An enzyme-linked immunosorbent assay is used to measure albumin in the bound and nonbound fractions of an aminophenylboronic acid chromatographic separation. The fingerstick method for assay of glycosylated plasma albumin gives results that are ~40% higher than comparable values obtained on the same patient with a 1-ml plasma sample determined with the bromcresol green technique. There is good correlation of fingerstick glycoalbumins with fingerstick glycohemoglobins and glycosylated plasma protein values. These procedures should be useful for children and for large-scale ambulatory screening for diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)629-632
Number of pages4
JournalDiabetes Care
Volume10
Issue number5
DOIs
StatePublished - Jan 1 1987

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Advanced and Specialized Nursing

Fingerprint Dive into the research topics of 'Fingerstick glycosylated hemoglobin, plasma protein, and albumin'. Together they form a unique fingerprint.

  • Cite this

    Rendell, M., Brannan, C., Nierenberg, J., Rasbold, K., & Hestorff, T. (1987). Fingerstick glycosylated hemoglobin, plasma protein, and albumin. Diabetes Care, 10(5), 629-632. https://doi.org/10.2337/diacare.10.5.629