Flow Cytometry Analysis of Fungal Ploidy

Robert T. Todd, Ann L. Braverman, Anna Selmecki

Research output: Contribution to journalArticle

Abstract

Ploidy, the number of sets of homologous chromosomes in a cell, can alter cellular physiology, gene regulation, and the spectrum of acquired mutations. Advances in single-cell flow cytometry have greatly improved the understanding of how genome size contributes to diverse biological processes including speciation, adaptation, pathogenesis, and tumorigenesis. For example, fungal pathogens can undergo whole genome duplications during infection of the human host and during acquisition of antifungal drug resistance. Quantification of ploidy is dramatically affected by the nucleic acid staining technique and the flow cytometry analysis of single cells. Ploidy in fungi is also impacted by samples that are heterogeneous for both ploidy and morphology, and control strains with known ploidy must be included in every flow cytometry experiment. To detect ploidy changes within fungal strains, the following protocol was developed to accurately and dependably interrogate single-cell ploidy.

Original languageEnglish (US)
Article numbere58
JournalCurrent Protocols in Microbiology
Volume50
Issue number1
DOIs
StatePublished - Aug 1 2018

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Ploidies
Flow Cytometry
Fungal Drug Resistance
Single-Cell Analysis
Biological Phenomena
Genome Size
Nucleic Acids
Carcinogenesis
Fungi
Chromosomes
Genome
Staining and Labeling
Mutation
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Virology

Cite this

Flow Cytometry Analysis of Fungal Ploidy. / Todd, Robert T.; Braverman, Ann L.; Selmecki, Anna.

In: Current Protocols in Microbiology, Vol. 50, No. 1, e58, 01.08.2018.

Research output: Contribution to journalArticle

Todd, Robert T. ; Braverman, Ann L. ; Selmecki, Anna. / Flow Cytometry Analysis of Fungal Ploidy. In: Current Protocols in Microbiology. 2018 ; Vol. 50, No. 1.
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