Abstract
The nanosecond time-resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D-glucose can decrease the fluorescence lifetimes, while L-glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D-glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L-glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D-glucose are different from those induced by L-glucose.
Original language | English (US) |
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Pages (from-to) | 279-284 |
Number of pages | 6 |
Journal | Biochemistry and Molecular Biology International |
Volume | 39 |
Issue number | 2 |
DOIs | |
State | Published - 1996 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Genetics