Abstract
The nanosecond time-resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D-glucose can decrease the fluorescence lifetimes, while L-glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D-glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L-glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D-glucose are different from those induced by L-glucose.
| Original language | English |
|---|---|
| Pages (from-to) | 279-284 |
| Number of pages | 6 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 39 |
| Issue number | 2 |
| State | Published - 1996 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Genetics
- Molecular Biology
Cite this
Fluorescence study on ligand induced conformational changes of human erythrocyte glucose transporter. / Xie, W. S.; Yue, J. C.; Tu, Yaping.
In: Biochemistry and Molecular Biology International, Vol. 39, No. 2, 1996, p. 279-284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluorescence study on ligand induced conformational changes of human erythrocyte glucose transporter
AU - Xie, W. S.
AU - Yue, J. C.
AU - Tu, Yaping
PY - 1996
Y1 - 1996
N2 - The nanosecond time-resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D-glucose can decrease the fluorescence lifetimes, while L-glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D-glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L-glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D-glucose are different from those induced by L-glucose.
AB - The nanosecond time-resolved fluorescence and fluorescence lifetime quenching have been used to detect the conformational changes of human erythrocyte glucose transporter induced by its ligands. Results show that D-glucose can decrease the fluorescence lifetimes, while L-glucose exhibits no effect. The fluorescence lifetime quenching results also show that in the presence of D-glucose, the quenching efficiency of hypocrellin B (a hydrophobic quencher obtained from a parasitic fungus, growing in Yunnan, China) is lower than in the presence of L-glucose. It can be deduced that the conformational changes of human erythrocyte glucose transporter induced by D-glucose are different from those induced by L-glucose.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029954465&partnerID=8YFLogxK
M3 - Article
C2 - 8799454
AN - SCOPUS:0029954465
VL - 39
SP - 279
EP - 284
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 2
ER -