Frequent emergence and limited geographic dispersal of methicillin- resistant Staphylococcus aureus

Ulrich Nübel, Philippe Roumagnac, Mirjam Feldkamp, Jae Hoon Song, Kwan Soo Ko, Yhu Chering Huang, Geoffrey Coombs, Margaret Ip, Henrik Westh, Robert Skov, Marc J. Struelens, Richard V. Goering, Birgit Strommenger, Annette Weller, Wolfgang Witte, Mark Achtman

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Abstract

A small number of clonal lineages dominates the global population structure of methicillin-resistant Staphylococcus aureus (MRSA), resulting in the concept that MRSA has emerged on a few occasions after penicillinase-stable β-lactam antibiotics were introduced to clinical practice, followed by intercontinental spread of individual clones. We investigated the evolutionary history of an MRSA clone (ST5) by mutation discovery at 108 loci (46 kb) within a global collection of 135 isolates. The SNPs that were ascertained define a radial phylogenetic structure within ST5 consisting of at least 5 chains of mutational steps that define geographically associated clades. These clades are not concordant with previously described groupings based on staphylococcal protein A gene (spa) typing. By mapping the number of independent imports of the staphylococcal cassette chromosome methicillin-resistance island, we also show that import has occurred on at least 23 occasions within this single sequence type and that the progeny of such recombinant strains usually are distributed locally rather than globally. These results provide strong evidence that geographical spread of MRSA over long distances and across cultural borders is a rare event compared with the frequency with which the staphylococcal cassette chromosome island has been imported.

Original languageEnglish
Pages (from-to)14130-14135
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number37
DOIs
StatePublished - Sep 16 2008

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Methicillin-Resistant Staphylococcus aureus
Clone Cells
Chromosomes
Penicillinase
Lactams
Methicillin Resistance
Staphylococcal Protein A
Islands
Single Nucleotide Polymorphism
History
Anti-Bacterial Agents
Mutation
Population
Genes

All Science Journal Classification (ASJC) codes

  • General

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Frequent emergence and limited geographic dispersal of methicillin- resistant Staphylococcus aureus. / Nübel, Ulrich; Roumagnac, Philippe; Feldkamp, Mirjam; Song, Jae Hoon; Ko, Kwan Soo; Huang, Yhu Chering; Coombs, Geoffrey; Ip, Margaret; Westh, Henrik; Skov, Robert; Struelens, Marc J.; Goering, Richard V.; Strommenger, Birgit; Weller, Annette; Witte, Wolfgang; Achtman, Mark.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 37, 16.09.2008, p. 14130-14135.

Research output: Contribution to journalArticle

Nübel, U, Roumagnac, P, Feldkamp, M, Song, JH, Ko, KS, Huang, YC, Coombs, G, Ip, M, Westh, H, Skov, R, Struelens, MJ, Goering, RV, Strommenger, B, Weller, A, Witte, W & Achtman, M 2008, 'Frequent emergence and limited geographic dispersal of methicillin- resistant Staphylococcus aureus', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 37, pp. 14130-14135. https://doi.org/10.1073/pnas.0804178105
Nübel, Ulrich ; Roumagnac, Philippe ; Feldkamp, Mirjam ; Song, Jae Hoon ; Ko, Kwan Soo ; Huang, Yhu Chering ; Coombs, Geoffrey ; Ip, Margaret ; Westh, Henrik ; Skov, Robert ; Struelens, Marc J. ; Goering, Richard V. ; Strommenger, Birgit ; Weller, Annette ; Witte, Wolfgang ; Achtman, Mark. / Frequent emergence and limited geographic dispersal of methicillin- resistant Staphylococcus aureus. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 37. pp. 14130-14135.
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