G-proteins in rat blood vessels-II. Assessment of functional involvement

Worku Abebe, John D. Edwards, Devendra K. Agrawal

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Abstract

1. 1. In this study, we compared G-protein-mediated contractile responses of rat aorta and mesenteric artery rings induced by the α-adrenoceptor agonist, norepinephrine (NE) and by the direct G-protein activator, sodium fluoride, using various probes. 2. 2. Activator of the stimulatory G-protein (Gs), cholera toxin (CT), attenuated the sensitivity and maximum contractile response of both aorta and mesenteric artery to NE and sodium fluoride. The effect of the toxin on the NE-sensitivity was greater in mesenteric artery. 3. 3. Pretreatment of tissues with the inhibitor of Gi-protein, pertussis toxin (PT), reduced the sensitivity as well as maximum contraction of both the aorta and mesenteric artery to sodium fluoride, and of the mesenteric artery to NE. PT attenuated only the sensitivity but not the maximum contraction of the aorta to NE. The inhibitory effect of PT on sensitivity to NE or sodium fluoride was greater in the aorta. 4. 4. NE and sodium fluoride-induced contractions were reduced by the sulfhydryl G-protein inhibitor, N-ethylmaleimide (NEM) in both blood vessels. NEM produced greater inhibitory effect on the sensitivity of the aorta to both contractile agents. 5. 5. These data demonstrate that CT, PT and NEM-sensitive G-proteins are involved in NE- and sodium fluoride-induced contractile responses of the rat aorta and mesenteric artery. The differential effects of the G-protein probes indicate that certain variations in G-protein-mediated contractile responses exist among the two blood vessels, suggesting that G-protein involvement in functional responses may vary with the type of blood vessel investigated.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalGeneral Pharmacology
Volume26
Issue number1
DOIs
StatePublished - 1995

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GTP-Binding Proteins
Sodium Fluoride
Mesenteric Arteries
Blood Vessels
Norepinephrine
Aorta
Pertussis Toxin
Ethylmaleimide
Cholera Toxin
N-Ethylmaleimide-Sensitive Proteins
Gs GTP-Binding Protein alpha Subunits
Adrenergic Receptors
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology

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G-proteins in rat blood vessels-II. Assessment of functional involvement. / Abebe, Worku; Edwards, John D.; Agrawal, Devendra K.

In: General Pharmacology, Vol. 26, No. 1, 1995, p. 75-83.

Research output: Contribution to journalArticle

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