GABAA receptor subunits in the human amygdala and hippocampus

Immunohistochemical distribution of 7 subunits

Harald Stefanits, Ivan Milenkovic, Nina Mahr, Ekaterina Pataraia, Johannes A. Hainfellner, Gabor G. Kovacs, Werner Sieghart, Deniz Yilmazer-Hanke, Thomas Czech

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

GABAergic neurotransmission in the amygdala contributes to the regulation of emotional processes in anxiety, stress, reward, mnestic functions, addiction, and epilepsy. Species-specific differences in the distribution and composition of GABAA receptors may account for distinct effects and side-effects of GABAergic agents. However, data on the distribution and composition of GABAA receptors in the human amygdala are lacking. Here, the expression of GABAA receptor subunits α1, α2, α3, α5, β2, β2/3, and γ2 was studied in the human amygdala using immunohistochemistry. Hippocampi were evaluated as a reference structure. Neuronal counts and field fraction analyses were performed, and subcellular expression of GABAA receptor subunits was analyzed semiquantitatively. In the amygdala, field fraction analyses showed the highest α1 expression in the lateral nucleus (La), whereas α3 was prominent in intercalated nuclei (IC), and α5 and γ2 in the cortical nuclei, and amygdalo-hippocampal/parahippocampal-amygdala transition areas. In the hippocampus, α1 and α3 were accentuated in the dentate gyrus, CA1 region, and subiculum, whereas α5 expression was rather uniform. In both regions, α2 was homogenously distributed, and the two β subunits and γ2 showed faint immunostaining. The intensity of subunit expression also varied in the neuropil, neuronal somata, and/or cellular processes in the subregions. GABAA receptors containing subunit α1, showing the strongest expression in the La, and α3, with the strongest expression in the IC and subiculum, could be targets for treating amygdala-related disorders. Differences in GABAA receptor subunit expression between the human and rodent amygdala should be taken into consideration when developing subunit-selective drugs.

Original languageEnglish (US)
JournalJournal of Comparative Neurology
DOIs
StateAccepted/In press - 2017
Externally publishedYes

Fingerprint

GABA-A Receptors
Amygdala
Hippocampus
GABA Agents
Neuropil
Dentate Gyrus
Carisoprodol
Reward
Synaptic Transmission
Rodentia
Epilepsy
Anxiety
Immunohistochemistry
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Stefanits, H., Milenkovic, I., Mahr, N., Pataraia, E., Hainfellner, J. A., Kovacs, G. G., ... Czech, T. (Accepted/In press). GABAA receptor subunits in the human amygdala and hippocampus: Immunohistochemical distribution of 7 subunits. Journal of Comparative Neurology. https://doi.org/10.1002/cne.24337

GABAA receptor subunits in the human amygdala and hippocampus : Immunohistochemical distribution of 7 subunits. / Stefanits, Harald; Milenkovic, Ivan; Mahr, Nina; Pataraia, Ekaterina; Hainfellner, Johannes A.; Kovacs, Gabor G.; Sieghart, Werner; Yilmazer-Hanke, Deniz; Czech, Thomas.

In: Journal of Comparative Neurology, 2017.

Research output: Contribution to journalArticle

Stefanits, H, Milenkovic, I, Mahr, N, Pataraia, E, Hainfellner, JA, Kovacs, GG, Sieghart, W, Yilmazer-Hanke, D & Czech, T 2017, 'GABAA receptor subunits in the human amygdala and hippocampus: Immunohistochemical distribution of 7 subunits', Journal of Comparative Neurology. https://doi.org/10.1002/cne.24337
Stefanits, Harald ; Milenkovic, Ivan ; Mahr, Nina ; Pataraia, Ekaterina ; Hainfellner, Johannes A. ; Kovacs, Gabor G. ; Sieghart, Werner ; Yilmazer-Hanke, Deniz ; Czech, Thomas. / GABAA receptor subunits in the human amygdala and hippocampus : Immunohistochemical distribution of 7 subunits. In: Journal of Comparative Neurology. 2017.
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