Ganglioside GM3 modulates conformation of reconstituted Ca2+-ATPase

Lihua Wang, Xiaoyi Yang, Yaping Tu, Zhaochun Gui, Fuyu Yang

Research output: Contribution to journalArticle

Abstract

Using steady-state fluorescence and nanosecond time-resolved fluorescence techniques, the Ca2+-ATPase conformational changes induced by ganglioside GM3 were studied with different quenchers. The results showed that GM3 could significantly increase the lifetime of intrinsic fluorescence of Ca2+ -ATPase reconstituted into proteoliposomes, and could also weaken the intrinsic fluorescence quenching by KI or hypocrellin B, HB. Furthermore, by using quenching kinetic analysis of the time-resolved fluorescence, in the presence of GM3, the quenching constant (Ksv) and quenching efficiency were significantly lowered. The obtained results suggest that the oligosaccharide chain and the ceramide moieties of the GM3 molecule could interact with its counterparts of the Ca2+ -ATPase respectively, thus change the conformation of the hydrophobic domain of the enzyme, making the tryptophan residues in different regions shift towards the hydrophilic-hydrophobic interface, and hence shorten the distance between the hydrophilic and the hydrophobic domains, making the enzyme with a more compact form exhibit higher enzyme activity.

Original languageEnglish
Pages (from-to)422-429
Number of pages8
JournalScience in China, Series C: Life Sciences
Volume40
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

G(M3) Ganglioside
gangliosides
Ca2-transporting ATPase
Calcium-Transporting ATPases
Conformations
fluorescence
Fluorescence
Quenching
Enzymes
enzyme
ceramides
Ceramides
Enzyme activity
enzymes
Oligosaccharides
oligosaccharides
Tryptophan
tryptophan
enzyme activity
kinetics

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Ganglioside GM3 modulates conformation of reconstituted Ca2+-ATPase. / Wang, Lihua; Yang, Xiaoyi; Tu, Yaping; Gui, Zhaochun; Yang, Fuyu.

In: Science in China, Series C: Life Sciences, Vol. 40, No. 4, 1997, p. 422-429.

Research output: Contribution to journalArticle

Wang, Lihua ; Yang, Xiaoyi ; Tu, Yaping ; Gui, Zhaochun ; Yang, Fuyu. / Ganglioside GM3 modulates conformation of reconstituted Ca2+-ATPase. In: Science in China, Series C: Life Sciences. 1997 ; Vol. 40, No. 4. pp. 422-429.
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