Gastrin 1-6 promotes growth of colon cancer cells through non-CCK receptors

Jeffrey Copps, Shawn Ahmed, Richard F. Murphy, Sándor Lovas

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Our previous studies have shown that stimulation of proliferation of DLD-1 and HT29 human colonic cancer cells by nanomolar gastrin (G17) and carboxymethyl gastrin (G17Gly) and reversal of growth by micromolar G17 and G17Gly involves binding sites which can neither be CCK1 nor CCK2 receptors; the N terminal fragment, G17(1-12), is sufficient to increase the number of HT-29 cells by binding the higher affinity binding site but is without a suppressing effect through the lower affinity site. In this study with DLD-1 cells, competitive binding using 125I-G17(1-12) showed that G17(1-12) binds both high and low affinity sites, as do G17 and G17Gly. G17(1-6)-NH2, even without the central-to-C-terminal portion of G17, was still able to bind a single site and to promote a dose-dependent increase in cell number at nanomolar concentrations. The results indicate the presence of a non-CCK receptor on human colonic cancer cells which could mediate the tumor-promoting activity of the N-terminal-to-central portion of G17Gly which, unlike G17, is produced by such cells.

Original languageEnglish
Pages (from-to)632-635
Number of pages4
JournalPeptides
Volume28
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Gastrins
Colonic Neoplasms
Binding Sites
Cells
Cholecystokinin B Receptor
Growth
Tumors
HT29 Cells
Gastrin-Secreting Cells
Competitive Binding
Cell Count
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Gastrin 1-6 promotes growth of colon cancer cells through non-CCK receptors. / Copps, Jeffrey; Ahmed, Shawn; Murphy, Richard F.; Lovas, Sándor.

In: Peptides, Vol. 28, No. 3, 03.2007, p. 632-635.

Research output: Contribution to journalArticle

Copps, Jeffrey ; Ahmed, Shawn ; Murphy, Richard F. ; Lovas, Sándor. / Gastrin 1-6 promotes growth of colon cancer cells through non-CCK receptors. In: Peptides. 2007 ; Vol. 28, No. 3. pp. 632-635.
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