Genes interconnecting AMPK and TREM-1 and associated microRNAs in rotator cuff tendon injury

Finosh G. Thankam, Chandra S. Boosani, Matthew Dilisio, R. Michael Gross, Devendra K. Agrawal

Research output: Contribution to journalArticle

Abstract

Fatty infiltration and inflammation delay the healing responses and raise major concerns in the therapeutic management of rotator cuff tendon injuries (RCTI). Our evaluations showed the upregulation of ‘metabolic check point’ AMPK and inflammatory molecule, TREM-1 from shoulder biceps tendons collected from RCTI subjects. However, the epigenetic regulation of these biomolecules by miRNAs is largely unknown and it is likely that a deeper understanding of the mechanism of action can have therapeutic potential for RCTI. Based on this background, we have evaluated the miRNAs from RCTI patients with fatty infiltration and inflammation (FI group) and compared with RCTI patients without fatty infiltration and inflammation (No-FI group). NetworkAnalyst was employed to evaluate the genes interconnecting AMPK and TREM-1 pathway, using PRKAA1 (AMPK), TREM-1, HIF1α, HMGB1, and AGER as input genes. The most relevant miRNAs were screened by considering the fold change below − 7.5 and the number of target genes 10 and more which showed 13 miRNAs and 216 target genes. The exact role of these miRNAs in the fatty infiltration and inflammation associated with RCTI is still unknown and the understanding of biological activity of these miRNAs can pave ways to develop miRNA-based therapeutics in the management of RCTI.

Original languageEnglish (US)
JournalMolecular and Cellular Biochemistry
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Tendon Injuries
AMP-Activated Protein Kinases
Tendons
MicroRNAs
Genes
Infiltration
Inflammation
HMGB1 Protein
Rotator Cuff Injuries
Epigenomics
Biomolecules
Bioactivity
Up-Regulation
Therapeutics
Molecules

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Genes interconnecting AMPK and TREM-1 and associated microRNAs in rotator cuff tendon injury. / Thankam, Finosh G.; Boosani, Chandra S.; Dilisio, Matthew; Gross, R. Michael; Agrawal, Devendra K.

In: Molecular and Cellular Biochemistry, 01.01.2018.

Research output: Contribution to journalArticle

@article{105604540dfa40cf97477e4fdda7e1e8,
title = "Genes interconnecting AMPK and TREM-1 and associated microRNAs in rotator cuff tendon injury",
abstract = "Fatty infiltration and inflammation delay the healing responses and raise major concerns in the therapeutic management of rotator cuff tendon injuries (RCTI). Our evaluations showed the upregulation of ‘metabolic check point’ AMPK and inflammatory molecule, TREM-1 from shoulder biceps tendons collected from RCTI subjects. However, the epigenetic regulation of these biomolecules by miRNAs is largely unknown and it is likely that a deeper understanding of the mechanism of action can have therapeutic potential for RCTI. Based on this background, we have evaluated the miRNAs from RCTI patients with fatty infiltration and inflammation (FI group) and compared with RCTI patients without fatty infiltration and inflammation (No-FI group). NetworkAnalyst was employed to evaluate the genes interconnecting AMPK and TREM-1 pathway, using PRKAA1 (AMPK), TREM-1, HIF1α, HMGB1, and AGER as input genes. The most relevant miRNAs were screened by considering the fold change below − 7.5 and the number of target genes 10 and more which showed 13 miRNAs and 216 target genes. The exact role of these miRNAs in the fatty infiltration and inflammation associated with RCTI is still unknown and the understanding of biological activity of these miRNAs can pave ways to develop miRNA-based therapeutics in the management of RCTI.",
author = "Thankam, {Finosh G.} and Boosani, {Chandra S.} and Matthew Dilisio and Gross, {R. Michael} and Agrawal, {Devendra K.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/s11010-018-3456-z",
language = "English (US)",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Genes interconnecting AMPK and TREM-1 and associated microRNAs in rotator cuff tendon injury

AU - Thankam, Finosh G.

AU - Boosani, Chandra S.

AU - Dilisio, Matthew

AU - Gross, R. Michael

AU - Agrawal, Devendra K.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Fatty infiltration and inflammation delay the healing responses and raise major concerns in the therapeutic management of rotator cuff tendon injuries (RCTI). Our evaluations showed the upregulation of ‘metabolic check point’ AMPK and inflammatory molecule, TREM-1 from shoulder biceps tendons collected from RCTI subjects. However, the epigenetic regulation of these biomolecules by miRNAs is largely unknown and it is likely that a deeper understanding of the mechanism of action can have therapeutic potential for RCTI. Based on this background, we have evaluated the miRNAs from RCTI patients with fatty infiltration and inflammation (FI group) and compared with RCTI patients without fatty infiltration and inflammation (No-FI group). NetworkAnalyst was employed to evaluate the genes interconnecting AMPK and TREM-1 pathway, using PRKAA1 (AMPK), TREM-1, HIF1α, HMGB1, and AGER as input genes. The most relevant miRNAs were screened by considering the fold change below − 7.5 and the number of target genes 10 and more which showed 13 miRNAs and 216 target genes. The exact role of these miRNAs in the fatty infiltration and inflammation associated with RCTI is still unknown and the understanding of biological activity of these miRNAs can pave ways to develop miRNA-based therapeutics in the management of RCTI.

AB - Fatty infiltration and inflammation delay the healing responses and raise major concerns in the therapeutic management of rotator cuff tendon injuries (RCTI). Our evaluations showed the upregulation of ‘metabolic check point’ AMPK and inflammatory molecule, TREM-1 from shoulder biceps tendons collected from RCTI subjects. However, the epigenetic regulation of these biomolecules by miRNAs is largely unknown and it is likely that a deeper understanding of the mechanism of action can have therapeutic potential for RCTI. Based on this background, we have evaluated the miRNAs from RCTI patients with fatty infiltration and inflammation (FI group) and compared with RCTI patients without fatty infiltration and inflammation (No-FI group). NetworkAnalyst was employed to evaluate the genes interconnecting AMPK and TREM-1 pathway, using PRKAA1 (AMPK), TREM-1, HIF1α, HMGB1, and AGER as input genes. The most relevant miRNAs were screened by considering the fold change below − 7.5 and the number of target genes 10 and more which showed 13 miRNAs and 216 target genes. The exact role of these miRNAs in the fatty infiltration and inflammation associated with RCTI is still unknown and the understanding of biological activity of these miRNAs can pave ways to develop miRNA-based therapeutics in the management of RCTI.

UR - http://www.scopus.com/inward/record.url?scp=85055248020&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055248020&partnerID=8YFLogxK

U2 - 10.1007/s11010-018-3456-z

DO - 10.1007/s11010-018-3456-z

M3 - Article

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

ER -