Genetic load caused by variation in the amount of rDNA in a wasp

S. M.S.R. Araújo; C. C. Silva; S. G. Pompolo; F. Perfectti; J. P.M. Camacho

doi:10.1023/A:1020970820513

Genetic load caused by variation in the amount of rDNA in a wasp

S. M.S.R. Araújo, C. C. Silva, S. G. Pompolo, F. Perfectti, J. P.M. Camacho

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Abstract

Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.

Original language	English (US)
Pages (from-to)	607-613
Number of pages	7
Journal	Chromosome Research
Volume	10
Issue number	7
DOIs	https://doi.org/10.1023/A:1020970820513
State	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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@article{6e9b4b9077f348b893a875fd65fae02a,

title = "Genetic load caused by variation in the amount of rDNA in a wasp",

abstract = "Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.",

author = "Ara{\'u}jo, {S. M.S.R.} and Silva, {C. C.} and Pompolo, {S. G.} and F. Perfectti and Camacho, {J. P.M.}",

note = "Funding Information: We thank M. J. P. Arau¤ jo for his assistance in the collection of specimens and Dr. S. M. Recco-Pimentel (IB-UNICAMP) for supplying the pDm and her laboratory facilities. This work was supported by grants from CAPES and CNPq. F.P and J.P.M.C were supported by Spanish Direccio¤ n General de Investigacio¤ n (BOS2000^ 1521) and Plan Andaluz de Investigacio¤ n (Grupo CVI-165).",

year = "2002",

doi = "10.1023/A:1020970820513",

language = "English (US)",

volume = "10",

pages = "607--613",

journal = "Chromosome Research",

issn = "0967-3849",

publisher = "Springer Netherlands",

number = "7",

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T1 - Genetic load caused by variation in the amount of rDNA in a wasp

AU - Araújo, S. M.S.R.

AU - Silva, C. C.

AU - Pompolo, S. G.

AU - Perfectti, F.

AU - Camacho, J. P.M.

N1 - Funding Information: We thank M. J. P. Arau¤ jo for his assistance in the collection of specimens and Dr. S. M. Recco-Pimentel (IB-UNICAMP) for supplying the pDm and her laboratory facilities. This work was supported by grants from CAPES and CNPq. F.P and J.P.M.C were supported by Spanish Direccio¤ n General de Investigacio¤ n (BOS2000^ 1521) and Plan Andaluz de Investigacio¤ n (Grupo CVI-165).

PY - 2002

Y1 - 2002

N2 - Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.

AB - Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.

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Genetic load caused by variation in the amount of rDNA in a wasp

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