Abstract
Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.
| Original language | English |
|---|---|
| Pages (from-to) | 607-613 |
| Number of pages | 7 |
| Journal | Chromosome Research |
| Volume | 10 |
| Issue number | 7 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Genetics
Cite this
Genetic load caused by variation in the amount of rDNA in a wasp. / Rocha-Sanchez, Sonia; Silva, C. C.; Pompolo, S. G.; Perfectti, F.; Camacho, J. P M.
In: Chromosome Research, Vol. 10, No. 7, 2002, p. 607-613.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic load caused by variation in the amount of rDNA in a wasp
AU - Rocha-Sanchez, Sonia
AU - Silva, C. C.
AU - Pompolo, S. G.
AU - Perfectti, F.
AU - Camacho, J. P M
PY - 2002
Y1 - 2002
N2 - Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.
AB - Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.
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U2 - 10.1023/A:1020970820513
DO - 10.1023/A:1020970820513
M3 - Article
C2 - 12498349
AN - SCOPUS:0036447478
VL - 10
SP - 607
EP - 613
JO - Chromosome Research
JF - Chromosome Research
SN - 0967-3849
IS - 7
ER -