Genetic load caused by variation in the amount of rDNA in a wasp

S. M.S.R. Araújo; C. C. Silva; S. G. Pompolo; F. Perfectti; J. P.M. Camacho

doi:10.1023/A:1020970820513

Genetic load caused by variation in the amount of rDNA in a wasp

S. M.S.R. Araújo, C. C. Silva, S. G. Pompolo, F. Perfectti, J. P.M. Camacho

Department of Oral Biology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.

Original language	English (US)
Pages (from-to)	607-613
Number of pages	7
Journal	Chromosome Research
Volume	10
Issue number	7
DOIs	https://doi.org/10.1023/A:1020970820513
State	Published - 2002

All Science Journal Classification (ASJC) codes

Genetics

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@article{6e9b4b9077f348b893a875fd65fae02a,

title = "Genetic load caused by variation in the amount of rDNA in a wasp",

abstract = "Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.",

author = "Ara{\'u}jo, {S. M.S.R.} and Silva, {C. C.} and Pompolo, {S. G.} and F. Perfectti and Camacho, {J. P.M.}",

note = "Funding Information: We thank M. J. P. Arau¤ jo for his assistance in the collection of specimens and Dr. S. M. Recco-Pimentel (IB-UNICAMP) for supplying the pDm and her laboratory facilities. This work was supported by grants from CAPES and CNPq. F.P and J.P.M.C were supported by Spanish Direccio¤ n General de Investigacio¤ n (BOS2000^ 1521) and Plan Andaluz de Investigacio¤ n (Grupo CVI-165).",

year = "2002",

doi = "10.1023/A:1020970820513",

language = "English (US)",

volume = "10",

pages = "607--613",

journal = "Chromosome Research",

issn = "0967-3849",

publisher = "Springer Netherlands",

number = "7",

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AU - Araújo, S. M.S.R.

AU - Silva, C. C.

AU - Pompolo, S. G.

AU - Perfectti, F.

AU - Camacho, J. P.M.

N1 - Funding Information: We thank M. J. P. Arau¤ jo for his assistance in the collection of specimens and Dr. S. M. Recco-Pimentel (IB-UNICAMP) for supplying the pDm and her laboratory facilities. This work was supported by grants from CAPES and CNPq. F.P and J.P.M.C were supported by Spanish Direccio¤ n General de Investigacio¤ n (BOS2000^ 1521) and Plan Andaluz de Investigacio¤ n (Grupo CVI-165).

PY - 2002

Y1 - 2002

N2 - Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.

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