Glucose-Deprivation-Induced [3H]D-Aspartate Release from Isolated Bovine and Human Retinae

Sunny E. Ohia, S. Olubusayo Awe, Catherine A. Opere, Angela M. LeDay, Lydia C. Harris, Kaustubh Kulkarni, Najam A. Sharif

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The glucose deprivation-induced release of [3H]D-aspartate was studied in bovine and human retinas in a superfusion apparatus. [ 3H]D-aspartate release was significantly increased upon omitting glucose in the superfusion buffer. This effect was dependent on external Ca 2+ because L- and N-type Ca2+-channel blockers, such as diltiazem (1 μM), nitrendipine (1 μM), and ω-conotoxin (100 nM), significantly reduced the effect of glucose-deprivation induced release of [3H]D-aspartate. Furthermore, while glutamate receptor agonists (L-glutamate, N-methyl-D-aspartate, but not kainate) potentiated the effects of glucose deprivation, antagonists (MK-801, MCPG, ifenprodil, and L-AP3) at these receptors blocked the glucose deprivation-induced release process. Taken together, these studies have demonstrated that under conditions of glucose deprivation, as may happen during ischemic events in vivo, the retinal glutamatergic nerve endings and/or glial cells promote the efflux of [ 3H]D-aspartate into the extracellular environment. This process appears to be receptor-mediated and dependent on extracellular Ca2+ and is similar to previous reports pertaining to brain tissues.

Original languageEnglish
Pages (from-to)599-609
Number of pages11
JournalJournal of Ocular Pharmacology and Therapeutics
Volume19
Issue number6
StatePublished - Dec 2003

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D-Aspartic Acid
Retina
Glucose
Excitatory Amino Acid Agonists
Nitrendipine
Dizocilpine Maleate
Nerve Endings
Diltiazem
Kainic Acid
Glutamate Receptors
N-Methylaspartate
Neuroglia
Glutamic Acid
Buffers
Neurons
Brain

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Ohia, S. E., Awe, S. O., Opere, C. A., LeDay, A. M., Harris, L. C., Kulkarni, K., & Sharif, N. A. (2003). Glucose-Deprivation-Induced [3H]D-Aspartate Release from Isolated Bovine and Human Retinae. Journal of Ocular Pharmacology and Therapeutics, 19(6), 599-609.

Glucose-Deprivation-Induced [3H]D-Aspartate Release from Isolated Bovine and Human Retinae. / Ohia, Sunny E.; Awe, S. Olubusayo; Opere, Catherine A.; LeDay, Angela M.; Harris, Lydia C.; Kulkarni, Kaustubh; Sharif, Najam A.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 19, No. 6, 12.2003, p. 599-609.

Research output: Contribution to journalArticle

Ohia, SE, Awe, SO, Opere, CA, LeDay, AM, Harris, LC, Kulkarni, K & Sharif, NA 2003, 'Glucose-Deprivation-Induced [3H]D-Aspartate Release from Isolated Bovine and Human Retinae', Journal of Ocular Pharmacology and Therapeutics, vol. 19, no. 6, pp. 599-609.
Ohia, Sunny E. ; Awe, S. Olubusayo ; Opere, Catherine A. ; LeDay, Angela M. ; Harris, Lydia C. ; Kulkarni, Kaustubh ; Sharif, Najam A. / Glucose-Deprivation-Induced [3H]D-Aspartate Release from Isolated Bovine and Human Retinae. In: Journal of Ocular Pharmacology and Therapeutics. 2003 ; Vol. 19, No. 6. pp. 599-609.
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