Group B coxsackievirus diabetogenic phenotype correlates with replication efficiency

Toru Kanno, Kisoon Kim, Ken Kono, Kristen M. Drescher, Nora M. Chapman, Steven Tracy

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Group B coxsackieviruses can initiate rapid onset type 1 diabetes (T1D) in old nonobese diabetic (NOD) mice. Inoculating high doses of poorly pathogenic CVB3/GA per mouse initiated rapid onset T1D. Viral protein was detectable in islets shortly after inoculation in association with beta cells as well as other primary islet cell types. The virulent strain CVB3/28 replicated to higher titers more rapidly than CVB3/GA in the pancreas and in established beta cell cultures. Exchange of 5′-nontranslated regions between the two CVB3 strains demonstrated a variable impact on replication in beta cell cultures and suppression of in vivo replication for both strains. While any CVB strain may be able to induce T1D in prediabetic NOD mice, T1D onset is linked both to the viral replication rate and infectious dose.

Original languageEnglish
Pages (from-to)5637-5643
Number of pages7
JournalJournal of Virology
Volume80
Issue number11
DOIs
StatePublished - Jun 2006

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Human Enterovirus B
Enterovirus
insulin-dependent diabetes mellitus
Type 1 Diabetes Mellitus
Phenotype
phenotype
Inbred NOD Mouse
mice
cell culture
Cell Culture Techniques
viral proteins
islets of Langerhans
Viral Proteins
virus replication
dosage
Islets of Langerhans
pancreas
Pancreas
cells

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Group B coxsackievirus diabetogenic phenotype correlates with replication efficiency. / Kanno, Toru; Kim, Kisoon; Kono, Ken; Drescher, Kristen M.; Chapman, Nora M.; Tracy, Steven.

In: Journal of Virology, Vol. 80, No. 11, 06.2006, p. 5637-5643.

Research output: Contribution to journalArticle

Kanno, Toru ; Kim, Kisoon ; Kono, Ken ; Drescher, Kristen M. ; Chapman, Nora M. ; Tracy, Steven. / Group B coxsackievirus diabetogenic phenotype correlates with replication efficiency. In: Journal of Virology. 2006 ; Vol. 80, No. 11. pp. 5637-5643.
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