GTP increases airway muscarinic antagonist binding sites: An effect regulated by Mg2+

John B. Cheng, Robert G. Townley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Using [3H]QNB, we demonstrated that GTP and Gpp(NH)p increased muscarinic receptor high affinity sites in bovine tracheal muscle preparations; however, neither the dissociation constant of [3H]QNB binding sites nor pulmonary β-adrenergic receptor sites was altered. The GTP effect on increasing the receptor sites was relatively small (16%), sensitive (ED50 0.48 μM) and specific (Gpp(NH)p > GTP = GDP > GMP ≫ ATP). Mg2+ potentiated this increase by up to 94.8%, whereas Na+, K+ and Ca2+ had no such effect.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalEuropean Journal of Pharmacology
Volume88
Issue number2-3
DOIs
StatePublished - Mar 25 1983

Fingerprint

Muscarinic Antagonists
Guanosine Triphosphate
Guanylyl Imidodiphosphate
Binding Sites
Muscarinic Receptors
Adrenergic Receptors
Adenosine Triphosphate
Muscles
Lung

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

GTP increases airway muscarinic antagonist binding sites : An effect regulated by Mg2+. / Cheng, John B.; Townley, Robert G.

In: European Journal of Pharmacology, Vol. 88, No. 2-3, 25.03.1983, p. 269-272.

Research output: Contribution to journalArticle

Cheng, John B. ; Townley, Robert G. / GTP increases airway muscarinic antagonist binding sites : An effect regulated by Mg2+. In: European Journal of Pharmacology. 1983 ; Vol. 88, No. 2-3. pp. 269-272.
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