Guanine nucleotide binding regulatory proteins

Their characteristics and identification

Research output: Contribution to journalReview article

16 Citations (Scopus)

Abstract

Many biological signals are processed by the binding of chemicals to cell surface receptors. Signals are switched to intracellular language via guanine nucleotide binding regulatory proteins (G-proteins) which are present in all eukaryotic cells. Thus, G-proteins serve as interfaces between receptor-response coupling. Two forms of G-proteins have been reported: conventional G-proteins which are heterotrimeric and consist of α, β, and γ subunits, and monomeric small molecular weight G-proteins which are generally found as single polypeptides. Recently, high molecular weight G-proteins have also been described. The family of G-proteins contains multiple genes that encode the α, β, or γ subunits. G-proteins play a pivotal role in excitation-contraction coupling in smooth muscle function and control metabolic and secretory processes. In this review article, we have given a brief overview on the characteristics and methodology for the identification of G-proteins. The heterotrimeric G-proteins are generally identified by Western blotting and ADP-ribosylation with bacterial toxins. The monomeric and high molecular weight G-proteins have been identified by [35S]GTPδS overlay technique and photoaffinity labeling, respectively. Recently, the use of molecular genetic probes has made it possible to investigate the expression of the message for various G-proteins.

Original languageEnglish
Pages (from-to)187-196
Number of pages10
JournalJournal of Pharmacological and Toxicological Methods
Volume32
Issue number4
DOIs
StatePublished - 1994

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Guanine Nucleotides
GTP-Binding Proteins
Carrier Proteins
Proteins
Molecular Weight
Molecular weight
Bacterial Toxins
Molecular Probes
Excitation Contraction Coupling
Secretory Pathway
Cell Surface Receptors
Eukaryotic Cells
Adenosine Diphosphate
Labeling
Smooth Muscle
Muscle
Molecular Biology
Language
Genes
Western Blotting

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology

Cite this

Guanine nucleotide binding regulatory proteins : Their characteristics and identification. / Ali, Nawab; Agrawal, Devendra K.

In: Journal of Pharmacological and Toxicological Methods, Vol. 32, No. 4, 1994, p. 187-196.

Research output: Contribution to journalReview article

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AB - Many biological signals are processed by the binding of chemicals to cell surface receptors. Signals are switched to intracellular language via guanine nucleotide binding regulatory proteins (G-proteins) which are present in all eukaryotic cells. Thus, G-proteins serve as interfaces between receptor-response coupling. Two forms of G-proteins have been reported: conventional G-proteins which are heterotrimeric and consist of α, β, and γ subunits, and monomeric small molecular weight G-proteins which are generally found as single polypeptides. Recently, high molecular weight G-proteins have also been described. The family of G-proteins contains multiple genes that encode the α, β, or γ subunits. G-proteins play a pivotal role in excitation-contraction coupling in smooth muscle function and control metabolic and secretory processes. In this review article, we have given a brief overview on the characteristics and methodology for the identification of G-proteins. The heterotrimeric G-proteins are generally identified by Western blotting and ADP-ribosylation with bacterial toxins. The monomeric and high molecular weight G-proteins have been identified by [35S]GTPδS overlay technique and photoaffinity labeling, respectively. Recently, the use of molecular genetic probes has made it possible to investigate the expression of the message for various G-proteins.

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