Heterogeneity in ampr-ampc gene interaction in enterobacter cloacae

Richard V. Goering, Christine C. Sanders, W. Eugene Sanders, Roger Guay, Sylvain Guerin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The ampR gene and its regulation of AmpC B-lactamase synthesis were investigated for Enterobacter cloacae 1194E, a wild-type strain producing a group A (pI 8.7) enzyme. Expression of the cloned E. cloacae 1194E ampR-ampC region was examined initially in Escherichia coli HBIOI. However, transformants showed only constitutive B-Iactamase expression. For study of enzyme expression in a more closely related host, the cloned E. cloacae 1194EampR-ampC region was transformed into E. cloacae 55, a wild-type strain producing a group B (pI 7.8) enzyme. Results indicated a functional E. cloacae 1194EampR gene that could not be transcomplemented by E. cloacae 55. A comparative analysis of ampR nucleotide and amino acid-sequence data from E. cloacae 1194E and E. cloacaeMHNI revealed related but divergent genes. Thermal induction studies of AmpC B-lactamase also indicated a difference between E. cloacae 1194E and E. cloacae 55 in ampR-ampC interaction. Thus, it appears that, in at least some strains of Enterobacter, significant intraspecies divergence of ampR has occurred. This heterogeneity in ampR would not have been detected with β-Iactamase expression studies conducted exclusively in E. coli.

Original languageEnglish
Pages (from-to)786-792
Number of pages7
JournalClinical Infectious Diseases
Volume10
Issue number4
DOIs
StatePublished - 1988

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Enterobacter cloacae
Genes
Enzymes
Escherichia coli
Enterobacter
Amino Acid Sequence
Nucleotides
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Heterogeneity in ampr-ampc gene interaction in enterobacter cloacae. / Goering, Richard V.; Sanders, Christine C.; Sanders, W. Eugene; Guay, Roger; Guerin, Sylvain.

In: Clinical Infectious Diseases, Vol. 10, No. 4, 1988, p. 786-792.

Research output: Contribution to journalArticle

Goering, Richard V. ; Sanders, Christine C. ; Sanders, W. Eugene ; Guay, Roger ; Guerin, Sylvain. / Heterogeneity in ampr-ampc gene interaction in enterobacter cloacae. In: Clinical Infectious Diseases. 1988 ; Vol. 10, No. 4. pp. 786-792.
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