Histamine utilizes JAK-STAT pathway in regulating cytokine production

Natalia Osna, Kathleen Elliott, Oleg Chaika, Eric Patterson, Robert E. Lewis, Manzoor M. Khan

Research output: Contribution to journalArticle

Abstract

Histamine shifts TH1/TH2 cytokine balance from TH1 to TH2 cytokines. The phosphorylation of STAT factors and their translocation to nucleus are important steps in the regulation of TH1/TH2 cytokine balance. This study was designed to investigate the effects of histamine on Janus kinases-signal transducers and activators of transcription (JAK-STAT) pathway. The splenocytes were treated with histamine in the presence or absence of JAK-STAT inhibitor, tyrphostin, activated with IFNγ for 30 min, and phosphorylated STAT1 was detected by immunoblotting. We found that histamine up-regulated the phosphorylation of STAT1 and tyrphostin prevented this phosphorylation. We then studied the effects of tyrphostin on histamine-mediated inhibition of IFNγ production and histamine-mediated stimulation of IL-5 and IL-10 production. Tyrphostin dose-dependently reversed the effects of histamine on IFNγ, IL-5 and IL-10 production, as evident by ELISA. These observations suggest that histamine regulated JAK-STAT signal transduction, which is involved in cytokine secretion.

Original languageEnglish (US)
Pages (from-to)759-762
Number of pages4
JournalInternational Immunopharmacology
Volume1
Issue number4
DOIs
StatePublished - Mar 22 2001

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Proto-Oncogene Proteins
Janus Kinase 2
STAT1 Transcription Factor
Inbred AKR Mouse
Janus Kinases
Trans-Activators
Interleukin-5
DNA-Binding Proteins
Transducers
Interleukin-10
Protein-Tyrosine Kinases
Histamine
Interferon-gamma
Phosphorylation
Tyrphostins
Cytokines
Immunoblotting
Signal Transduction
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Histamine utilizes JAK-STAT pathway in regulating cytokine production. / Osna, Natalia; Elliott, Kathleen; Chaika, Oleg; Patterson, Eric; Lewis, Robert E.; Khan, Manzoor M.

In: International Immunopharmacology, Vol. 1, No. 4, 22.03.2001, p. 759-762.

Research output: Contribution to journalArticle

Osna, Natalia ; Elliott, Kathleen ; Chaika, Oleg ; Patterson, Eric ; Lewis, Robert E. ; Khan, Manzoor M. / Histamine utilizes JAK-STAT pathway in regulating cytokine production. In: International Immunopharmacology. 2001 ; Vol. 1, No. 4. pp. 759-762.
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