Abstract
Elevated homocysteine (Hcys) increases the risk of neurocristopathies. Previous studies show Hcys inhibits neural crest (NC) cell migration in vivo. However, the mechanisms responsible for this effect are unknown. Here, we evaluated the effect of Hcys on NC cell attachment in vitro and determined if any of the effects were due to altered Ca2+ signaling. We found Hcys enhanced NC cell attachment in a dose and substrate-dependent manner. Ionomycin mimicked the effect of Hcys while BAPTA-AM and 2-APB blocked the effect of Hcys on NC attachment. In contrast, inhibitors of plasma membrane Ca2+ channels had no effect on NC attachment. Hcys also increased the emission of the intracellular Ca2+-sensitive probe, Fluo-4. These results show Hcys alters NC attachment by triggering an increase in intracellular Ca2+ possibly by generating inositol triphosphate. Hence, the teratogenic effect ascribed to Hcys may be due to perturbation of intracellular Ca2+ signaling.
| Original language | English |
|---|---|
| Pages (from-to) | 2117-2128 |
| Number of pages | 12 |
| Journal | Developmental Dynamics |
| Volume | 237 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2008 |
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All Science Journal Classification (ASJC) codes
- Cell Biology
- Developmental Biology
Cite this
Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels. / Heidenreich, David J.; Reedy, Mark; Brauer, Philip R.
In: Developmental Dynamics, Vol. 237, No. 8, 08.2008, p. 2117-2128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels
AU - Heidenreich, David J.
AU - Reedy, Mark
AU - Brauer, Philip R.
PY - 2008/8
Y1 - 2008/8
N2 - Elevated homocysteine (Hcys) increases the risk of neurocristopathies. Previous studies show Hcys inhibits neural crest (NC) cell migration in vivo. However, the mechanisms responsible for this effect are unknown. Here, we evaluated the effect of Hcys on NC cell attachment in vitro and determined if any of the effects were due to altered Ca2+ signaling. We found Hcys enhanced NC cell attachment in a dose and substrate-dependent manner. Ionomycin mimicked the effect of Hcys while BAPTA-AM and 2-APB blocked the effect of Hcys on NC attachment. In contrast, inhibitors of plasma membrane Ca2+ channels had no effect on NC attachment. Hcys also increased the emission of the intracellular Ca2+-sensitive probe, Fluo-4. These results show Hcys alters NC attachment by triggering an increase in intracellular Ca2+ possibly by generating inositol triphosphate. Hence, the teratogenic effect ascribed to Hcys may be due to perturbation of intracellular Ca2+ signaling.
AB - Elevated homocysteine (Hcys) increases the risk of neurocristopathies. Previous studies show Hcys inhibits neural crest (NC) cell migration in vivo. However, the mechanisms responsible for this effect are unknown. Here, we evaluated the effect of Hcys on NC cell attachment in vitro and determined if any of the effects were due to altered Ca2+ signaling. We found Hcys enhanced NC cell attachment in a dose and substrate-dependent manner. Ionomycin mimicked the effect of Hcys while BAPTA-AM and 2-APB blocked the effect of Hcys on NC attachment. In contrast, inhibitors of plasma membrane Ca2+ channels had no effect on NC attachment. Hcys also increased the emission of the intracellular Ca2+-sensitive probe, Fluo-4. These results show Hcys alters NC attachment by triggering an increase in intracellular Ca2+ possibly by generating inositol triphosphate. Hence, the teratogenic effect ascribed to Hcys may be due to perturbation of intracellular Ca2+ signaling.
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U2 - 10.1002/dvdy.21644
DO - 10.1002/dvdy.21644
M3 - Article
VL - 237
SP - 2117
EP - 2128
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 8
ER -