Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels

David J. Heidenreich, Mark Reedy, Philip R. Brauer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Elevated homocysteine (Hcys) increases the risk of neurocristopathies. Previous studies show Hcys inhibits neural crest (NC) cell migration in vivo. However, the mechanisms responsible for this effect are unknown. Here, we evaluated the effect of Hcys on NC cell attachment in vitro and determined if any of the effects were due to altered Ca2+ signaling. We found Hcys enhanced NC cell attachment in a dose and substrate-dependent manner. Ionomycin mimicked the effect of Hcys while BAPTA-AM and 2-APB blocked the effect of Hcys on NC attachment. In contrast, inhibitors of plasma membrane Ca2+ channels had no effect on NC attachment. Hcys also increased the emission of the intracellular Ca2+-sensitive probe, Fluo-4. These results show Hcys alters NC attachment by triggering an increase in intracellular Ca2+ possibly by generating inositol triphosphate. Hence, the teratogenic effect ascribed to Hcys may be due to perturbation of intracellular Ca2+ signaling.

Original languageEnglish
Pages (from-to)2117-2128
Number of pages12
JournalDevelopmental Dynamics
Volume237
Issue number8
DOIs
StatePublished - Aug 2008

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Neural Crest
Homocysteine
Calcium
Ionomycin
Inositol
Ion Channels
Cell Movement
In Vitro Techniques
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Developmental Biology

Cite this

Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels. / Heidenreich, David J.; Reedy, Mark; Brauer, Philip R.

In: Developmental Dynamics, Vol. 237, No. 8, 08.2008, p. 2117-2128.

Research output: Contribution to journalArticle

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