Abstract
Elevated homocysteine increases the risk of neurocristopathies. Here, we determined whether elevating homocysteine altered the proliferation or number of chick neural crest cells that form between the midotic and third somite in vivo. Homocysteine increased the number of neural tube cells but decreased neural crest cell number. However, the sum total of cells was not different from controls. In controls, the 5-bromo-2′-deoxyuridine-labeling index was higher in newly formed neural crest cells than in their progenitors, paralleling reports showing these progenitors must pass the restriction point before undergoing epithelial-mesenchymal transition. Homocysteine decreased the labeling index of newly formed neural crest cells, suggesting that it inhibited cell cycle progression of neural crest progenitors or the S-phase entry of newly formed neural crest cells. Homocysteine also inhibited neural crest dispersal and decreased the distance they migrated from the neural tube. These results show neural crest morphogenesis is directly altered by elevated homocysteine in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 63-73 |
| Number of pages | 11 |
| Journal | Developmental Dynamics |
| Volume | 229 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2004 |
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All Science Journal Classification (ASJC) codes
- Developmental Biology
- Cell Biology
Cite this
Homocysteine Inhibits Cardiac Neural Crest Cell Formation and Morphogenesis In Vivo. / Tierney, Brent J.; Ho, Trang; Reedy, Mark; Brauer, Philip R.
In: Developmental Dynamics, Vol. 229, No. 1, 01.2004, p. 63-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Homocysteine Inhibits Cardiac Neural Crest Cell Formation and Morphogenesis In Vivo
AU - Tierney, Brent J.
AU - Ho, Trang
AU - Reedy, Mark
AU - Brauer, Philip R.
PY - 2004/1
Y1 - 2004/1
N2 - Elevated homocysteine increases the risk of neurocristopathies. Here, we determined whether elevating homocysteine altered the proliferation or number of chick neural crest cells that form between the midotic and third somite in vivo. Homocysteine increased the number of neural tube cells but decreased neural crest cell number. However, the sum total of cells was not different from controls. In controls, the 5-bromo-2′-deoxyuridine-labeling index was higher in newly formed neural crest cells than in their progenitors, paralleling reports showing these progenitors must pass the restriction point before undergoing epithelial-mesenchymal transition. Homocysteine decreased the labeling index of newly formed neural crest cells, suggesting that it inhibited cell cycle progression of neural crest progenitors or the S-phase entry of newly formed neural crest cells. Homocysteine also inhibited neural crest dispersal and decreased the distance they migrated from the neural tube. These results show neural crest morphogenesis is directly altered by elevated homocysteine in vivo.
AB - Elevated homocysteine increases the risk of neurocristopathies. Here, we determined whether elevating homocysteine altered the proliferation or number of chick neural crest cells that form between the midotic and third somite in vivo. Homocysteine increased the number of neural tube cells but decreased neural crest cell number. However, the sum total of cells was not different from controls. In controls, the 5-bromo-2′-deoxyuridine-labeling index was higher in newly formed neural crest cells than in their progenitors, paralleling reports showing these progenitors must pass the restriction point before undergoing epithelial-mesenchymal transition. Homocysteine decreased the labeling index of newly formed neural crest cells, suggesting that it inhibited cell cycle progression of neural crest progenitors or the S-phase entry of newly formed neural crest cells. Homocysteine also inhibited neural crest dispersal and decreased the distance they migrated from the neural tube. These results show neural crest morphogenesis is directly altered by elevated homocysteine in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0346786474&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346786474&partnerID=8YFLogxK
U2 - 10.1002/dvdy.10469
DO - 10.1002/dvdy.10469
M3 - Article
C2 - 14699578
AN - SCOPUS:0346786474
VL - 229
SP - 63
EP - 73
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 1
ER -