Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 Vpx-mediated preintegration complex

Xiaogang Cheng, Michael A. Belshan, Lee Ratner

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Human immunodeficiency virus type 2 (HIV-2) Vpx is required for nuclear translocation of the viral preintegration complex (PIC) in quiescent cells. In order to decipher the mechanism of action of Vpx, a cDNA library was screened with the yeast two-hybrid assay, resulting in the identification of heat shock protein 40, Hsp40/DnaJB6, as a Vpx-interactive protein. Interaction with Vpx was confirmed by glutathione S-transferase (GST) pull-down and coimmunoprecipitation assays. Overexpression of Hsp40/DnaJB6 enhanced Vpx nuclear import, whereas overexpression of a nuclear localization mutant of Hsp40/DnaJB6 (H31Q) or down-regulation of Hsp40/DnaJB6 by small interfering RNA (siRNA) reduced the nuclear import of Vpx. Hsp40/DnaJB6 competed with the Pr55 Gag precursor protein for the binding of Vpx and incorporation into virus-like particles. Overexpression of Hsp40/DnaJB6 promoted viral PIC nuclear import, whereas siRNA down-regulation of Hsp40/DnaJB6 inhibited PIC nuclear import. These results demonstrate a role for Hsp40/DnaJB6 in the regulation of HIV-2 PIC nuclear transport.

Original languageEnglish
Pages (from-to)1229-1237
Number of pages9
JournalJournal of Virology
Volume82
Issue number3
DOIs
StatePublished - Feb 2008

Fingerprint

Human immunodeficiency virus 2
HIV-2
Cell Nucleus Active Transport
imports
small interfering RNA
Small Interfering RNA
HSP40 Heat-Shock Proteins
Down-Regulation
two hybrid system techniques
virus-like particles
protein binding
gag Gene Products
heat shock proteins
glutathione transferase
Two-Hybrid System Techniques
cDNA libraries
mechanism of action
Glutathione Transferase
Gene Library
Protein Binding

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 Vpx-mediated preintegration complex. / Cheng, Xiaogang; Belshan, Michael A.; Ratner, Lee.

In: Journal of Virology, Vol. 82, No. 3, 02.2008, p. 1229-1237.

Research output: Contribution to journalArticle

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