Human pheochromocytoma phenol sulfotransferase

biochemical properties and activities of thermolabile and thermostable forms

Erica G. Sinsheimer, Robert J. Anderson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To determine whether pheochromocytoma phenol sulfotransferase (PST) activities were similar to blood platelet PST activities, we established assay conditions and biochemical properties for the human pheochromocytoma enzymes. At least two forms of PST were present in high speed supernatant (HSS) preparations of the tumors. A thermolabile form (TL) and a thermostable form (TS) were similar to those of human platelet PST with regard to pH optima, apparent Km values, responses to 2,6-dichloro-4-nitrophenol and thermal stability. PST activities were measured in 74 tumors of neuroectodermal origin that had been stored at -80° C for a mean of 37.9 months. Levels of TL and TS PST activities decreased in a nonlinear fashion with time of sample storage. TL and TS PST activities of 4 samples assayed after 1.08 ± 1.95 (mean ± SD) month of storage were 167 ± 73 and 3110 ± 1817 U/mg protein, respectively (mean ± Sem). Our results indicated that the TL and TS forms of PST in pheochromocytoma HSS preparations were biochemically similar to platelet PST activities.

Original languageEnglish
Pages (from-to)55-70
Number of pages16
JournalClinica Chimica Acta
Volume164
Issue number1
DOIs
StatePublished - Apr 15 1987

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Arylsulfotransferase
Pheochromocytoma
Platelets
Blood Platelets
Tumors
Neuroectodermal Tumors
Assays
Thermodynamic stability
Blood
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry

Cite this

Human pheochromocytoma phenol sulfotransferase : biochemical properties and activities of thermolabile and thermostable forms. / Sinsheimer, Erica G.; Anderson, Robert J.

In: Clinica Chimica Acta, Vol. 164, No. 1, 15.04.1987, p. 55-70.

Research output: Contribution to journalArticle

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