Abstract
Thermolabile (TL) and thermostable (TS) forms of human platelet phenol sulfotransferase (PST) were measured over various periods of time. TL PST was assayed with dopamine as the substrate and TS PST was measured with phenol and with p-nitrophenol. Levels of TL and TS PST from the same subjects did not change significantly over 1 day, 1 week, 4 weeks, and 8.5 months. Interassay coefficients of variation of PST activities over 3-4 weeks measured in samples from 8-12 subjects with dopamine, phenol and p-nitrophenol were 13.96%, 13.60% and 13.30%, respectively. There was a significant correlation between PST activity measured in the same samples with phenol and with p-nitrophenol (r = 0.946, n = 18, p <0.0001). TS PST activity measured with p-nitrophenol was significantly higher (p <0.0001) in platelets from 51 black subjects than the level of TS PST in samples from 52 white subjects (0.72 ± 0.06 vs 0.42 ± 0.04 units/108 platelets, respectively; mean ± SEM). These results demonstrate the 'stability' of the levels of both forms of the enzyme with time. They also point out the usefulness of a reproducible assay for the detection of a racial difference in the levels of TS PST.
| Original language | English |
|---|---|
| Pages (from-to) | 185-196 |
| Number of pages | 12 |
| Journal | Clinica Chimica Acta |
| Volume | 138 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 13 1984 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Clinical Biochemistry
Cite this
Human platelet phenol sulfotransferase : stability of two forms of the enzyme with time and presence of a racial difference. / Anderson, Robert J.; Jackson, Bertha L.
In: Clinica Chimica Acta, Vol. 138, No. 2, 13.04.1984, p. 185-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human platelet phenol sulfotransferase
T2 - stability of two forms of the enzyme with time and presence of a racial difference
AU - Anderson, Robert J.
AU - Jackson, Bertha L.
PY - 1984/4/13
Y1 - 1984/4/13
N2 - Thermolabile (TL) and thermostable (TS) forms of human platelet phenol sulfotransferase (PST) were measured over various periods of time. TL PST was assayed with dopamine as the substrate and TS PST was measured with phenol and with p-nitrophenol. Levels of TL and TS PST from the same subjects did not change significantly over 1 day, 1 week, 4 weeks, and 8.5 months. Interassay coefficients of variation of PST activities over 3-4 weeks measured in samples from 8-12 subjects with dopamine, phenol and p-nitrophenol were 13.96%, 13.60% and 13.30%, respectively. There was a significant correlation between PST activity measured in the same samples with phenol and with p-nitrophenol (r = 0.946, n = 18, p <0.0001). TS PST activity measured with p-nitrophenol was significantly higher (p <0.0001) in platelets from 51 black subjects than the level of TS PST in samples from 52 white subjects (0.72 ± 0.06 vs 0.42 ± 0.04 units/108 platelets, respectively; mean ± SEM). These results demonstrate the 'stability' of the levels of both forms of the enzyme with time. They also point out the usefulness of a reproducible assay for the detection of a racial difference in the levels of TS PST.
AB - Thermolabile (TL) and thermostable (TS) forms of human platelet phenol sulfotransferase (PST) were measured over various periods of time. TL PST was assayed with dopamine as the substrate and TS PST was measured with phenol and with p-nitrophenol. Levels of TL and TS PST from the same subjects did not change significantly over 1 day, 1 week, 4 weeks, and 8.5 months. Interassay coefficients of variation of PST activities over 3-4 weeks measured in samples from 8-12 subjects with dopamine, phenol and p-nitrophenol were 13.96%, 13.60% and 13.30%, respectively. There was a significant correlation between PST activity measured in the same samples with phenol and with p-nitrophenol (r = 0.946, n = 18, p <0.0001). TS PST activity measured with p-nitrophenol was significantly higher (p <0.0001) in platelets from 51 black subjects than the level of TS PST in samples from 52 white subjects (0.72 ± 0.06 vs 0.42 ± 0.04 units/108 platelets, respectively; mean ± SEM). These results demonstrate the 'stability' of the levels of both forms of the enzyme with time. They also point out the usefulness of a reproducible assay for the detection of a racial difference in the levels of TS PST.
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U2 - 10.1016/0009-8981(84)90233-X
DO - 10.1016/0009-8981(84)90233-X
M3 - Article
VL - 138
SP - 185
EP - 196
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 2
ER -