Human platelet phenol sulphotransferase

Assay procedure, substrate and tissue correlations

Robert J. Anderson, Richard M. Weinshilboum, Sidney F. Phillips, Daniel D. Broughton

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Procedures for the precise assay of human platelet phenol sulphotransferase activity were determined. The coefficient of variation of the assay was 5.8% when the enzyme activity was expressed per 108 platelets, and was 9.4% when it was expressed per mg soluble platelet protein. Mean platelet phenol sulphotransferase (PST) activity in samples from 102 randomly selected adults was 1.2 ± 0.4 units/108 platelets (mean ± S.D.), with a range from 0.2 to 2.9. The mean activity for umbilical cord blood platelet PST was 0.93 ± 0.3 units/108 platelets (mean ± S.D., n = 27). The substrate used routinely for the assay was 3-methoxy-4-hydroxyphenylglycol (MHPG). There was a significant correlation between the formation of MHPG sulfate by individual platelet preparations and the formation of sulfated product with each of the following substrates: tyramine (r = 0.92, n = 21); dopamine (r = 0.82, n = 16); 5-hydroxytryptamine (r = 0.94, n = 20); acetaminophen (r = 0.77, n = 17); and alphamethyldopa (r = 0.77, n = 17) (p <0.001 for each). Platelet PST activity correlated significantly with human renal cortex PST activity (r = 0.54, n = 20, p <0.02). The correlation coefficient between platelet PST activity and jejunal mucosal enzyme activity in eight patients was 0.67. These results raise the possibility that human platelet PST activity measured with MHPG as substrate might reflect the enzyme activity in other tissues and the degree of sulfate conjugation of a variety of substrates.

Original languageEnglish
Pages (from-to)157-167
Number of pages11
JournalClinica Chimica Acta
Volume110
Issue number2-3
DOIs
StatePublished - Mar 5 1981
Externally publishedYes

Fingerprint

Arylsulfotransferase
Platelets
Assays
Blood Platelets
Tissue
Substrates
Enzyme activity
Sulfates
Enzymes
Methyldopa
Tyramine
Acetaminophen
Fetal Blood

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry

Cite this

Human platelet phenol sulphotransferase : Assay procedure, substrate and tissue correlations. / Anderson, Robert J.; Weinshilboum, Richard M.; Phillips, Sidney F.; Broughton, Daniel D.

In: Clinica Chimica Acta, Vol. 110, No. 2-3, 05.03.1981, p. 157-167.

Research output: Contribution to journalArticle

Anderson, Robert J. ; Weinshilboum, Richard M. ; Phillips, Sidney F. ; Broughton, Daniel D. / Human platelet phenol sulphotransferase : Assay procedure, substrate and tissue correlations. In: Clinica Chimica Acta. 1981 ; Vol. 110, No. 2-3. pp. 157-167.
@article{1143896463e84966bf890d684ec7ef8c,
title = "Human platelet phenol sulphotransferase: Assay procedure, substrate and tissue correlations",
abstract = "Procedures for the precise assay of human platelet phenol sulphotransferase activity were determined. The coefficient of variation of the assay was 5.8{\%} when the enzyme activity was expressed per 108 platelets, and was 9.4{\%} when it was expressed per mg soluble platelet protein. Mean platelet phenol sulphotransferase (PST) activity in samples from 102 randomly selected adults was 1.2 ± 0.4 units/108 platelets (mean ± S.D.), with a range from 0.2 to 2.9. The mean activity for umbilical cord blood platelet PST was 0.93 ± 0.3 units/108 platelets (mean ± S.D., n = 27). The substrate used routinely for the assay was 3-methoxy-4-hydroxyphenylglycol (MHPG). There was a significant correlation between the formation of MHPG sulfate by individual platelet preparations and the formation of sulfated product with each of the following substrates: tyramine (r = 0.92, n = 21); dopamine (r = 0.82, n = 16); 5-hydroxytryptamine (r = 0.94, n = 20); acetaminophen (r = 0.77, n = 17); and alphamethyldopa (r = 0.77, n = 17) (p <0.001 for each). Platelet PST activity correlated significantly with human renal cortex PST activity (r = 0.54, n = 20, p <0.02). The correlation coefficient between platelet PST activity and jejunal mucosal enzyme activity in eight patients was 0.67. These results raise the possibility that human platelet PST activity measured with MHPG as substrate might reflect the enzyme activity in other tissues and the degree of sulfate conjugation of a variety of substrates.",
author = "Anderson, {Robert J.} and Weinshilboum, {Richard M.} and Phillips, {Sidney F.} and Broughton, {Daniel D.}",
year = "1981",
month = "3",
day = "5",
doi = "10.1016/0009-8981(81)90345-4",
language = "English",
volume = "110",
pages = "157--167",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Human platelet phenol sulphotransferase

T2 - Assay procedure, substrate and tissue correlations

AU - Anderson, Robert J.

AU - Weinshilboum, Richard M.

AU - Phillips, Sidney F.

AU - Broughton, Daniel D.

PY - 1981/3/5

Y1 - 1981/3/5

N2 - Procedures for the precise assay of human platelet phenol sulphotransferase activity were determined. The coefficient of variation of the assay was 5.8% when the enzyme activity was expressed per 108 platelets, and was 9.4% when it was expressed per mg soluble platelet protein. Mean platelet phenol sulphotransferase (PST) activity in samples from 102 randomly selected adults was 1.2 ± 0.4 units/108 platelets (mean ± S.D.), with a range from 0.2 to 2.9. The mean activity for umbilical cord blood platelet PST was 0.93 ± 0.3 units/108 platelets (mean ± S.D., n = 27). The substrate used routinely for the assay was 3-methoxy-4-hydroxyphenylglycol (MHPG). There was a significant correlation between the formation of MHPG sulfate by individual platelet preparations and the formation of sulfated product with each of the following substrates: tyramine (r = 0.92, n = 21); dopamine (r = 0.82, n = 16); 5-hydroxytryptamine (r = 0.94, n = 20); acetaminophen (r = 0.77, n = 17); and alphamethyldopa (r = 0.77, n = 17) (p <0.001 for each). Platelet PST activity correlated significantly with human renal cortex PST activity (r = 0.54, n = 20, p <0.02). The correlation coefficient between platelet PST activity and jejunal mucosal enzyme activity in eight patients was 0.67. These results raise the possibility that human platelet PST activity measured with MHPG as substrate might reflect the enzyme activity in other tissues and the degree of sulfate conjugation of a variety of substrates.

AB - Procedures for the precise assay of human platelet phenol sulphotransferase activity were determined. The coefficient of variation of the assay was 5.8% when the enzyme activity was expressed per 108 platelets, and was 9.4% when it was expressed per mg soluble platelet protein. Mean platelet phenol sulphotransferase (PST) activity in samples from 102 randomly selected adults was 1.2 ± 0.4 units/108 platelets (mean ± S.D.), with a range from 0.2 to 2.9. The mean activity for umbilical cord blood platelet PST was 0.93 ± 0.3 units/108 platelets (mean ± S.D., n = 27). The substrate used routinely for the assay was 3-methoxy-4-hydroxyphenylglycol (MHPG). There was a significant correlation between the formation of MHPG sulfate by individual platelet preparations and the formation of sulfated product with each of the following substrates: tyramine (r = 0.92, n = 21); dopamine (r = 0.82, n = 16); 5-hydroxytryptamine (r = 0.94, n = 20); acetaminophen (r = 0.77, n = 17); and alphamethyldopa (r = 0.77, n = 17) (p <0.001 for each). Platelet PST activity correlated significantly with human renal cortex PST activity (r = 0.54, n = 20, p <0.02). The correlation coefficient between platelet PST activity and jejunal mucosal enzyme activity in eight patients was 0.67. These results raise the possibility that human platelet PST activity measured with MHPG as substrate might reflect the enzyme activity in other tissues and the degree of sulfate conjugation of a variety of substrates.

UR - http://www.scopus.com/inward/record.url?scp=0019450758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019450758&partnerID=8YFLogxK

U2 - 10.1016/0009-8981(81)90345-4

DO - 10.1016/0009-8981(81)90345-4

M3 - Article

VL - 110

SP - 157

EP - 167

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 2-3

ER -